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LearnA700-049
antibody from Invitrogen Antibodies
Targeting: SIRPA
BIT, CD172a, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A700-049 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIRP alpha Recombinant Rabbit Monoclonal Antibody (BLR049F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR049F
- Vial size
- 100 µL
- Concentration
- 250 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SIRP alpha by western blot. Samples: Whole cell lysate (50 µg) from Malme-3M, HeLa, 786-O, HEK293T, U2OS, OVCAR-4, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (Product # A700-049 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin (Product # A700-057).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SIRP alpha by immunocytochemistry.Sample: FFPE section of Malme-3M cells.Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (Product # A700-049; lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SIRP alpha by immunohistochemistry. Antibody Sample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-SIRP alpha recombinant monoclonal antibody (BLR049F) (A700-049 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SIRP alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-MEL-28 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SIRP alpha recombinant monoclonal antibody [BLR049F] (Product # A700-049 lot 1) used for IP at 20 µL per reaction. SIRP alpha was also immunoprecipitated by rabbit anti-SIRP alpha recombinant monoclonal antibody [BL-565-1D6]. For blotting immunoprecipitated SIRP alpha (Product # A700-049) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
