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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1035 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-19 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-19 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9UHD0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Potentiation of IL-19 expression in airway epithelia by IL-17A and IL-4/IL-13: important implications in asthma.
Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
European journal of immunology 2009 Oct;39(10):2779-88
European journal of immunology 2009 Oct;39(10):2779-88
Potentiation of IL-19 expression in airway epithelia by IL-17A and IL-4/IL-13: important implications in asthma.
Huang F, Wachi S, Thai P, Loukoianov A, Tan KH, Forteza RM, Wu R
The Journal of allergy and clinical immunology 2008 Jun;121(6):1415-21, 1421.e1-3
The Journal of allergy and clinical immunology 2008 Jun;121(6):1415-21, 1421.e1-3
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-19 and Neutralization by Human IL-19 Antibody. Recombinant Human IL-19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 R alpha and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1035). The ND50 is typically 1-3 µg/mL.
