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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF4828 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse VAMP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse VAMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VAMP-2 and less than 1% cross-reactivity with rhVAMP-5 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P23763- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
Forrest CM, Khalil OS, Pisar M, Smith RA, Darlington LG, Stone TW
Molecular brain 2012 Jun 9;5:22
Molecular brain 2012 Jun 9;5:22
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse VAMP-1 by Western Blot. Western blot shows lysates of human heart and brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VAMP-1 in Mouse Spinal Cord. VAMP-1 was detected in perfusion fixed frozen sections of mouse spinal cord using 1.7 µg/mL Goat Anti-Human/Mouse VAMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4828) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
