15047-1-AP

antibody from Proteintech Group

Targeting: TYMS HsT422, TMS, TS, Tsase

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

15047-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15047-1-AP, RRID:AB_2210721

Product name

Thymidylate synthase antibody

Antibody type

Polyclonal

Description

KD/KO validated Thymidylate synthase antibody (Cat. #15047-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A Novel Integrated Metabolism-Immunity Gene Expression Model Predicts the Prognosis of Lung Adenocarcinoma Patients.

Chen S, Duan Y, Wu Y, Yang D, An J
Frontiers in pharmacology 2021;12:728368

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Identification and Validation of TYMS as a Potential Biomarker for Risk of Metastasis Development in Hepatocellular Carcinoma.

Li S, Zhao J, Lv L, Dong D
Frontiers in oncology 2021;11:762821

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Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.

Chang SC, Yuan SH, Li CY, Chang HM, Wang HC, Pan YA, Hsueh PC, Wu CC, Yang Y, Liu HP
Veterinary and comparative oncology 2021 Mar;19(1):172-182

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CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.

Liang YY, Bacanu S, Sreekumar L, Ramos AD, Dai L, Michaelis M, Cinatl J, Seki T, Cao Y, Coffill CR, Lane DP, Prabhu N, Nordlund P
Cell chemical biology 2021 Jul 13;

---

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767

---

Uncovering the anti-NSCLC effects and mechanisms of gypenosides by metabolomics and network pharmacology analysis.

Qi YS, Xie JB, Xie P, Duan Y, Ling YQ, Gu YL, Piao XL
Journal of ethnopharmacology 2021 Dec 5;281:114506

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5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.

Zhao Y, Feng X, Chen Y, Selfridge JE, Gorityala S, Du Z, Wang JM, Hao Y, Cioffi G, Conlon RA, Barnholtz-Sloan JS, Saltzman J, Krishnamurthi SS, Vinayak S, Veigl M, Xu Y, Bajor DL, Markowitz SD, Meropol NJ, Eads JR, Wang Z
Cancer research 2020 Nov 1;80(21):4815-4827

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MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer.

Xu F, Ye ML, Zhang YP, Li WJ, Li MT, Wang HZ, Qiu X, Xu Y, Yin JW, Hu Q, Wei WH, Chang Y, Liu L, Zhao Q
Cancer science 2020 May;111(5):1528-1541

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Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Fu F, Zhang Y, Gao Z, Zhao Y, Wen Z, Han H, Li Y, Chen H
International journal of cancer 2020 Jul 15;147(2):584-592

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Pemetrexed inhibits Kaposi's sarcoma-associated herpesvirus replication through blocking dTMP synthesis.

Chen J, Zhang H, Chen X
Antiviral research 2020 Aug;180:104825

---

Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Xie W, Wang B, Wang X, Hou D, Su H, Huang H
Annals of translational medicine 2020 Apr;8(7):478

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MEK inhibitor enhanced the antitumor effect of oxaliplatin and 5‑fluorouracil in MEK1 Q56P‑mutant colorectal cancer cells.

Jing C, Li H, Du Y, Cao H, Liu S, Wang Z, Ma R, Feng J, Wu J
Molecular medicine reports 2019 Feb;19(2):1092-1100

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An efficient proteome-wide strategy for discovery and characterization of cellular nucleotide-protein interactions.

Lim YT, Prabhu N, Dai L, Go KD, Chen D, Sreekumar L, Egeblad L, Eriksson S, Chen L, Veerappan S, Teo HL, Tan CSH, Lengqvist J, Larsson A, Sobota RM, Nordlund P
PloS one 2018;13(12):e0208273

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The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346

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Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells.

Liang X, Shi H, Yang L, Qiu C, Lin S, Qi Y, Li J, Zhao A, Liu J
British journal of cancer 2017 Mar 28;116(7):903-911

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CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.

Almqvist H, Axelsson H, Jafari R, Dan C, Mateus A, Haraldsson M, Larsson A, Martinez Molina D, Artursson P, Lundbäck T, Nordlund P
Nature communications 2016 Mar 24;7:11040

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Suberoylanilide hydroxamic acid enhances chemosensitivity to 5-fluorouracil in hepatocellular carcinoma via inhibition of thymidylate synthase.

Liao B, Liang H, Chen J, Liu Q, Zhang B, Chen X
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Dec;36(12):9347-56

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Screening for Target Engagement using the Cellular Thermal Shift Assay - CETSA

Axelsson H, Almqvist H, Seashore-Ludlow B, Lundbäck T, Markossian S, Grossman A, Brimacombe K, Arkin M, Auld D, Austin CP, Baell J, Chung TDY, Coussens NP, Dahlin JL, Devanarayan V, Foley TL, Glicksman M, Hall MD, Haas JV, Hoare SRJ, Inglese J, Iversen PW, Kales SC, Lal-Nag M, Li Z, McGee J, McManus O, Riss T, Saradjian P, Sittampalam GS, Tarselli M, Trask OJ Jr., Wang Y, Weidner JR, Wildey MJ, Wilson K, Xia M, Xu X
2004;

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15047-1-AP(TYMS antibody) at dilution of 1:1500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using TYMS antibody 15047-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human endometrial cancer using 15047-1-AP(TYMS antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human endometrial cancer using 15047-1-AP(TYMS antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue