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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A300-794A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DC8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DC8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DC8 (Cat. No. A300-794A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DC8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti- DC8 antibody A300-794A (lot A300-794A-2) used for IP at 6 µg per reaction. DC8 was also immunoprecipitated by rabbit anti-DC8 antibody A300-795A. For blotting immunoprecipitated DC8, A300-795A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
