AP2035e

antibody from Abgent

Targeting: PROX1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2035e

Provider

Abgent

Proper citation

Abgent Cat#AP2035e, RRID:AB_10663178

Product name

PROX-1-S514 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysates from HepG2 cell line and mouse liver tissue lysate(from left to right), using PROX-1 Antibody (S514) (Cat. #AP2035e). AP2035e was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

PROX-1-S514 Antibody (Cat. #AP2035e) IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PROX-1-S514 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

PROX-1-S514 Antibody (Cat. #AP2035e) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50