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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB4458 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PTPN14 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human PTPN14 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PTPN13 or rhPTPN22.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q15678.2- Isotype
- IgG
- Antibody clone number
- 448701
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protein-tyrosine Phosphatase and Kinase Specificity in Regulation of SRC and Breast Tumor Kinase.
A novel phosphatidic acid-protein-tyrosine phosphatase D2 axis is essential for ERBB2 signaling in mammary epithelial cells.
Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells.
YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14.
Fan G, Aleem S, Yang M, Miller WT, Tonks NK
The Journal of biological chemistry 2015 Jun 26;290(26):15934-47
The Journal of biological chemistry 2015 Jun 26;290(26):15934-47
A novel phosphatidic acid-protein-tyrosine phosphatase D2 axis is essential for ERBB2 signaling in mammary epithelial cells.
Ramesh M, Krishnan N, Muthuswamy SK, Tonks NK
The Journal of biological chemistry 2015 Apr 10;290(15):9646-59
The Journal of biological chemistry 2015 Apr 10;290(15):9646-59
Protein-tyrosine phosphatase 1B antagonized signaling by insulin-like growth factor-1 receptor and kinase BRK/PTK6 in ovarian cancer cells.
Fan G, Lin G, Lucito R, Tonks NK
The Journal of biological chemistry 2013 Aug 23;288(34):24923-34
The Journal of biological chemistry 2013 Aug 23;288(34):24923-34
YAP modifies cancer cell sensitivity to EGFR and survivin inhibitors and is negatively regulated by the non-receptor type protein tyrosine phosphatase 14.
Huang JM, Nagatomo I, Suzuki E, Mizuno T, Kumagai T, Berezov A, Zhang H, Karlan B, Greene MI, Wang Q
Oncogene 2013 Apr 25;32(17):2220-9
Oncogene 2013 Apr 25;32(17):2220-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PTPN14 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PTPN14 in Human Placenta. PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
