Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- APC-047-GP-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KCNK2 (TREK-1) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Guinea Pig
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1,2. Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:800).3,4. Guinea pig Anti-KCNK2 (TREK-1) Antibody , preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of KCNK2 (TREK-1) in rat midbrain - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Guinea pig Anti-KCNK2 (TREK-1) Antibody (#APC-047-GP), (1:400), followed by goat- Anti-guinea pig-Cy3 Antibody . TREK-1 staining (red) appears in neurons in the rat lateral substantia nigra (arrows). Nuclei are stained with DAPI (blue).