MAB3059

antibody from R&D Systems

Targeting: S100A8 60B8AG, CAGA, CFAG, CGLA, MRP8, P8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3059

Provider

R&D Systems

Product name

Mouse S100A8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P27005


Isotype

IgG

Antibody clone number

335806

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development.

Makthal N, Nguyen K, Do H, Gavagan M, Chandrangsu P, Helmann JD, Olsen RJ, Kumaraswami M
EBioMedicine 2017 Jul;21:131-141

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The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.

Dessing MC, Tammaro A, Pulskens WP, Teske GJ, Butter LM, Claessen N, van Eijk M, van der Poll T, Vogl T, Roth J, Florquin S, Leemans JC
Kidney international 2015 Jan;87(1):85-94

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Visualized macrophage dynamics and significance of S100A8 in obese fat.

Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
Proceedings of the National Academy of Sciences of the United States of America 2015 Apr 21;112(16):E2058-66

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The acute neutrophil response mediated by S100 alarmins during vaginal Candida infections is independent of the Th17-pathway.

Yano J, Kolls JK, Happel KI, Wormley F, Wozniak KL, Fidel PL Jr
PloS one 2012;7(9):e46311

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S100A8/A9 is not involved in host defense against murine urinary tract infection.

Dessing MC, Butter LM, Teske GJ, Claessen N, van der Loos CM, Vogl T, Roth J, van der Poll T, Florquin S, Leemans JC
PloS one 2010 Oct 14;5(10):e13394

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.