HPA056863

antibody from Atlas Antibodies

Targeting: DSG3 CDHF6

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA056863

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA056863, RRID:AB_2683260

Product name

Anti-DSG3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTN
KAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNR
DSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPD
FND


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human esophagus and stomach tissues using Anti-DSG3 antibody. Corresponding DSG3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, esophagus, lymph node and testis using Anti-DSG3 antibody HPA056863 (A) shows similar protein distribution across tissues to independent antibody HPA046864 (B).

Antibody #2 product nr

HPA046864

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows strong membranous and cytoplasmic positivity in squamous epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-DSG3 antibody HPA056863.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-DSG3 antibody HPA056863.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-DSG3 antibody HPA056863.

Sample type

HUMAN