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- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- V3246 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Desmoglein 3 Antibody / DSG3
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5G11
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Desmoglein 3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Pathogenicity and Epitope Characteristics Do Not Differ in IgG Subclass-Switched Anti-Desmoglein 3 IgG1 and IgG4 Autoantibodies in Pemphigus Vulgaris.
Expression of desmosomal proteins in acantholytic squamous cell carcinoma of the skin.
Adducin is required for desmosomal cohesion in keratinocytes.
De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells.
MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris.
Immunohistochemical profiling of ALK fusion gene-positive adenocarcinomas of the lung.
Clinicopathological features of thymic carcinomas and the impact of histopathological agreement on prognostical studies.
Decreased expression of intercellular adhesion molecules in acantholytic squamous cell carcinoma compared with invasive well-differentiated squamous cell carcinoma of the skin.
Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.
Mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides.
Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.
Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays.
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity.
Investigation of human keratinocyte cell adhesion using atomic force microscopy.
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Severe keratin 5 and 14 mutations induce down-regulation of junction proteins in keratinocytes.
The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung.
Living skin equivalents constructed using human amnions as a matrix.
Sequential development of intercellular junctions in bioengineered human corneas.
Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies.
P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells.
Lo AS, Mao X, Mukherjee EM, Ellebrecht CT, Yu X, Posner MR, Payne AS, Cavacini LA
PloS one 2016 Jun 15;11(6):e0156800
PloS one 2016 Jun 15;11(6):e0156800
Expression of desmosomal proteins in acantholytic squamous cell carcinoma of the skin.
Jurčić V, Kukovič J, Zidar N
Histology and histopathology 2015 Aug;30(8):945-53
Histology and histopathology 2015 Aug;30(8):945-53
Adducin is required for desmosomal cohesion in keratinocytes.
Rötzer V, Breit A, Waschke J, Spindler V
The Journal of biological chemistry 2014 May 23;289(21):14925-40
The Journal of biological chemistry 2014 May 23;289(21):14925-40
De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells.
Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Oliveira C, Braziulis E, Klar AS, Meuli-Simmen C, Meuli M, Reichmann E
The Journal of investigative dermatology 2014 Jun;134(6):1735-42
The Journal of investigative dermatology 2014 Jun;134(6):1735-42
MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris.
Mao X, Li H, Sano Y, Gaestel M, Mo Park J, Payne AS
The Journal of investigative dermatology 2014 Jan;134(1):68-76
The Journal of investigative dermatology 2014 Jan;134(1):68-76
Immunohistochemical profiling of ALK fusion gene-positive adenocarcinomas of the lung.
Sakai Y, Nakai T, Ohbayashi C, Imagawa N, Yanagita E, Satake R, Nitta A, Kajimoto K, Sakuma T, Itoh T
International journal of surgical pathology 2013 Oct;21(5):476-82
International journal of surgical pathology 2013 Oct;21(5):476-82
Clinicopathological features of thymic carcinomas and the impact of histopathological agreement on prognostical studies.
Roden AC, Yi ES, Cassivi SD, Jenkins SM, Garces YI, Aubry MC
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2013 Jun;43(6):1131-9
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2013 Jun;43(6):1131-9
Decreased expression of intercellular adhesion molecules in acantholytic squamous cell carcinoma compared with invasive well-differentiated squamous cell carcinoma of the skin.
Griffin JR, Wriston CC, Peters MS, Lehman JS
American journal of clinical pathology 2013 Apr;139(4):442-7
American journal of clinical pathology 2013 Apr;139(4):442-7
Assessment of desmosomal components (desmoglein 1-3, plakoglobin) in cardia mucosa in relation to gastroesophageal reflux disease and Helicobacter pylori infection.
Wex T, Kuester D, Mönkemüller K, Stahr A, Fry LC, Kandulski A, Kropf S, Roessner A, Malfertheiner P
Human pathology 2012 Oct;43(10):1745-54
Human pathology 2012 Oct;43(10):1745-54
Mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides.
Dworschak J, Recke A, Freitag M, Ludwig RJ, Langenhan J, Kreuzer OJ, Zillikens D, Schmidt E
Journal of dermatological science 2012 Feb;65(2):102-9
Journal of dermatological science 2012 Feb;65(2):102-9
Gastro-oesophageal reflux disease is associated with up-regulation of desmosomal components in oesophageal mucosa.
Wex T, Mönkemüller K, Stahr A, Kuester D, Fry LC, Völkel S, Kandulski A, Roessner A, Malfertheiner P
Histopathology 2012 Feb;60(3):405-15
Histopathology 2012 Feb;60(3):405-15
Inhibition of CD44 gene expression in human skin models, using self-delivery short interfering RNA administered by dissolvable microneedle arrays.
Lara MF, González-González E, Speaker TJ, Hickerson RP, Leake D, Milstone LM, Contag CH, Kaspar RL
Human gene therapy 2012 Aug;23(8):816-23
Human gene therapy 2012 Aug;23(8):816-23
Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
Katafiasz D, Smith LM, Wahl JK 3rd
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity.
Schlegel N, Meir M, Heupel WM, Holthöfer B, Leube RE, Waschke J
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G774-83
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G774-83
Investigation of human keratinocyte cell adhesion using atomic force microscopy.
Fung CK, Seiffert-Sinha K, Lai KW, Yang R, Panyard D, Zhang J, Xi N, Sinha AA
Nanomedicine : nanotechnology, biology, and medicine 2010 Feb;6(1):191-200
Nanomedicine : nanotechnology, biology, and medicine 2010 Feb;6(1):191-200
Human eccrine sweat gland cells can reconstitute a stratified epidermis.
Biedermann T, Pontiggia L, Böttcher-Haberzeth S, Tharakan S, Braziulis E, Schiestl C, Meuli M, Reichmann E
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
The Journal of investigative dermatology 2010 Aug;130(8):1996-2009
Severe keratin 5 and 14 mutations induce down-regulation of junction proteins in keratinocytes.
Liovic M, D'Alessandro M, Tomic-Canic M, Bolshakov VN, Coats SE, Lane EB
Experimental cell research 2009 Oct 15;315(17):2995-3003
Experimental cell research 2009 Oct 15;315(17):2995-3003
The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung.
Savci-Heijink CD, Kosari F, Aubry MC, Caron BL, Sun Z, Yang P, Vasmatzis G
The American journal of pathology 2009 May;174(5):1629-37
The American journal of pathology 2009 May;174(5):1629-37
Living skin equivalents constructed using human amnions as a matrix.
Yang L, Shirakata Y, Tokumaru S, Xiuju D, Tohyama M, Hanakawa Y, Hirakawa S, Sayama K, Hashimoto K
Journal of dermatological science 2009 Dec;56(3):188-95
Journal of dermatological science 2009 Dec;56(3):188-95
Sequential development of intercellular junctions in bioengineered human corneas.
González-Andrades M, Garzón I, Gascón MI, Muñoz-Avila JI, Sánchez-Quevedo MC, Campos A, Alaminos M
Journal of tissue engineering and regenerative medicine 2009 Aug;3(6):442-9
Journal of tissue engineering and regenerative medicine 2009 Aug;3(6):442-9
Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies.
Mao X, Choi EJ, Payne AS
The Journal of investigative dermatology 2009 Apr;129(4):908-18
The Journal of investigative dermatology 2009 Apr;129(4):908-18
P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells.
Kanno M, Aoyama Y, Isa Y, Yamamoto Y, Kitajima Y
The Journal of dermatology 2008 Jun;35(6):317-24
The Journal of dermatology 2008 Jun;35(6):317-24
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skin with Desmoglein 3 antibody (clone 5G11). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Desmoglein 3 Antibody (clone 5G11). Confirmation of Integrity and Purity of the Antibody.
