NBP1-78984

antibody from Novus Biologicals

Targeting: DSG3 CDHF6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-78984

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-78984, RRID:AB_11021709

Product name

Mouse Monoclonal Desmoglein-3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Different signaling patterns contribute to loss of keratinocyte cohesion dependent on autoantibody profile in pemphigus.

Walter E, Vielmuth F, Rotkopf L, Sárdy M, Horváth ON, Goebeler M, Schmidt E, Eming R, Hertl M, Spindler V, Waschke J
Scientific reports 2017 Jun 15;7(1):3579

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Desmoglein 3, via an interaction with E-cadherin, is associated with activation of Src.

Tsang SM, Liu L, Teh MT, Wheeler A, Grose R, Hart IR, Garrod DR, Fortune F, Wan H
PloS one 2010 Dec 3;5(12):e14211

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Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases.

Mahoney MG, Hu Y, Brennan D, Bazzi H, Christiano AM, Wahl JK 3rd
Experimental dermatology 2006 Feb;15(2):101-9

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Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10.

Lucchese A, Mittelman A, Lin MS, Kanduc D, Sinha AA
Journal of translational medicine 2004 Dec 11;2(1):43

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Generation of monoclonal antibodies specific for desmoglein family members.

Wahl JK 3rd
Hybridoma and hybridomics 2002 Feb;21(1):37-44

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The fate of desmosomal proteins in apoptotic cells.

Weiske J, Schöneberg T, Schröder W, Hatzfeld M, Tauber R, Huber O
The Journal of biological chemistry 2001 Nov 2;276(44):41175-81

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Development of chimeric molecules for recognition and targeting of antigen-specific B cells in pemphigus vulgaris.

Proby CM, Ota T, Suzuki H, Koyasu S, Gamou S, Shimizu N, Wahl JK, Wheelock MJ, Nishikawa T, Amagai M
The British journal of dermatology 2000 Feb;142(2):321-30

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.