MA5-16025
antibody from Invitrogen Antibodies
Targeting: DSG3
CDHF6
Antibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [6]
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- Product number
- MA5-16025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Desmoglein 3 Monoclonal Antibody (5G11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes.
A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.
Desmosome assembly and disassembly are membrane raft-dependent.
Adducin is required for desmosomal cohesion in keratinocytes.
De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells.
Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma.
Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes.
MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris.
p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris.
Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity.
Autoimmunity to desmocollin 3 in pemphigus vulgaris.
Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies.
P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells.
Characterization of extracellular matrix components in the limbal epithelial stem cell compartment.
New skin-equivalent model from de-epithelialized amnion membrane.
Expression of desmosomal proteins in squamous cell carcinomas of the skin.
Expression of desmosomal proteins in squamous cell carcinomas of the skin.
Koch PJ, Webb S, Gugger JA, Salois MN, Koster MI
Current protocols 2022 Apr;2(4):e408
Current protocols 2022 Apr;2(4):e408
A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.
Ebert LM, Tan LY, Johan MZ, Min KK, Cockshell MP, Parham KA, Betterman KL, Szeto P, Boyle S, Silva L, Peng A, Zhang Y, Ruszkiewicz A, Zannettino AC, Gronthos S, Koblar S, Harvey NL, Lopez AF, Shackleton M, Bonder CS
Angiogenesis 2016 Oct;19(4):463-86
Angiogenesis 2016 Oct;19(4):463-86
Desmosome assembly and disassembly are membrane raft-dependent.
Stahley SN, Saito M, Faundez V, Koval M, Mattheyses AL, Kowalczyk AP
PloS one 2014;9(1):e87809
PloS one 2014;9(1):e87809
Adducin is required for desmosomal cohesion in keratinocytes.
Rötzer V, Breit A, Waschke J, Spindler V
The Journal of biological chemistry 2014 May 23;289(21):14925-40
The Journal of biological chemistry 2014 May 23;289(21):14925-40
De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells.
Pontiggia L, Biedermann T, Böttcher-Haberzeth S, Oliveira C, Braziulis E, Klar AS, Meuli-Simmen C, Meuli M, Reichmann E
The Journal of investigative dermatology 2014 Jun;134(6):1735-1742
The Journal of investigative dermatology 2014 Jun;134(6):1735-1742
Altered expression and localization of desmoglein 3 in esophageal squamous cell carcinoma.
Fang WK, Chen B, Xu XE, Liao LD, Wu ZY, Wu JY, Shen J, Xu LY, Li EM
Acta histochemica 2014 Jun;116(5):803-9
Acta histochemica 2014 Jun;116(5):803-9
Desmoglein 2 compensates for desmoglein 3 but does not control cell adhesion via regulation of p38 mitogen-activated protein kinase in keratinocytes.
Hartlieb E, Rötzer V, Radeva M, Spindler V, Waschke J
The Journal of biological chemistry 2014 Jun 13;289(24):17043-53
The Journal of biological chemistry 2014 Jun 13;289(24):17043-53
MAPKAP kinase 2 (MK2)-dependent and -independent models of blister formation in pemphigus vulgaris.
Mao X, Li H, Sano Y, Gaestel M, Mo Park J, Payne AS
The Journal of investigative dermatology 2014 Jan;134(1):68-76
The Journal of investigative dermatology 2014 Jan;134(1):68-76
p38 MAPK activation is downstream of the loss of intercellular adhesion in pemphigus vulgaris.
Mao X, Sano Y, Park JM, Payne AS
The Journal of biological chemistry 2011 Jan 14;286(2):1283-91
The Journal of biological chemistry 2011 Jan 14;286(2):1283-91
Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity.
Schlegel N, Meir M, Heupel WM, Holthöfer B, Leube RE, Waschke J
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G774-83
American journal of physiology. Gastrointestinal and liver physiology 2010 May;298(5):G774-83
Autoimmunity to desmocollin 3 in pemphigus vulgaris.
Mao X, Nagler AR, Farber SA, Choi EJ, Jackson LH, Leiferman KM, Ishii N, Hashimoto T, Amagai M, Zone JJ, Payne AS
The American journal of pathology 2010 Dec;177(6):2724-30
The American journal of pathology 2010 Dec;177(6):2724-30
Disruption of desmosome assembly by monovalent human pemphigus vulgaris monoclonal antibodies.
Mao X, Choi EJ, Payne AS
The Journal of investigative dermatology 2009 Apr;129(4):908-18
The Journal of investigative dermatology 2009 Apr;129(4):908-18
P120 catenin is associated with desmogleins when desmosomes are assembled in high-Ca2+ medium but not when disassembled in low-Ca2+ medium in DJM-1 cells.
Kanno M, Aoyama Y, Isa Y, Yamamoto Y, Kitajima Y
The Journal of dermatology 2008 Jun;35(6):317-24
The Journal of dermatology 2008 Jun;35(6):317-24
Characterization of extracellular matrix components in the limbal epithelial stem cell compartment.
Schlötzer-Schrehardt U, Dietrich T, Saito K, Sorokin L, Sasaki T, Paulsson M, Kruse FE
Experimental eye research 2007 Dec;85(6):845-60
Experimental eye research 2007 Dec;85(6):845-60
New skin-equivalent model from de-epithelialized amnion membrane.
Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, Sayama K, Hamuro J, Hashimoto K
Cell and tissue research 2006 Oct;326(1):69-77
Cell and tissue research 2006 Oct;326(1):69-77
Expression of desmosomal proteins in squamous cell carcinomas of the skin.
Kurzen H, Münzing I, Hartschuh W
Journal of cutaneous pathology 2003 Nov;30(10):621-30
Journal of cutaneous pathology 2003 Nov;30(10):621-30
Expression of desmosomal proteins in squamous cell carcinomas of the skin.
Kurzen H, Münzing I, Hartschuh W
Journal of cutaneous pathology 2003 Nov;30(10):621-30
Journal of cutaneous pathology 2003 Nov;30(10):621-30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Desmoglein 3 using a monoclonal antibody (Product # MA5-16025).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Desmoglein 3 in A-431 cells. Samples were incubated in Desmoglein 3 monoclonal antibody (Product # MA5-16025).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Desmoglein 3 in human skin cancer. Samples were incubated in Desmoglein 3 monoclonal antibody (Product # MA5-16025) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Desmoglein 3 in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in Desmoglein 3 monoclonal antibody (Product # MA5-16025) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 6 Figure Expression of keratinocyte markers in iPSC-K. iPSC-K and primary human epidermal keratinocytes were exposed to 1.3 mM Ca 2+ for 48 hr. ( A-F ) Immunofluorescence staining and ( G ) Western blot analysis demonstrates normal expression and localization of desmosomal proteins (DSC3, DSG3) and an adherens junction protein (beta-catenin). Antibodies used for immunofluorescence staining are: DSG3 (clone 5H10; courtesy of Dr. James K Wahl III, PhD, University of Nebraska Medical Center, Lincoln, NE), DSC3 (Progen cat. no. 61093), and beta-catenin (Santa Cruz cat. no. sc-7963). Antibodies used forlotting are: DSG3 (Invitrogen cat. no. MA5-16025), DSC3 (Progen cat. no. 61093), and beta- catenin (Santa Cruz cat. no. sc-7963).