Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-59947 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Lamin B Receptor Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human, mouse Lamin B Receptor antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Lamin B Receptor Antibody (BB2SS3F3) [NBP2-59947] - Analysis of whole cell lysate from human (Hela) and mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), NIE-115) cell lines were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB2SS3F3 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.