12398-1-AP

antibody from Invitrogen Antibodies

Targeting: LBR DHCR14B, TDRD18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12398-1-AP

Provider

Invitrogen Antibodies

Product name

LBR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPSRKFADG EVVRGRWPGS SLYYEVEILS HDSTSQLYTV KYKDGTELEL KENDIKPLTS FRQRKGGSTS SSPSRRRGSR SRSRSRSPGR PPKSARRSAS ASHQADIKEA RREVEVKLTP LILKPFGNSI SRYNGEPEHI ERNDAPHKNT QEKFSLSQES SYIATQYSLR PRREEVKLKE IDSKEEKYVA KELAVRTFEV TPIRAKDLEF G (1-210 aa encoded by BC020079)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C2C12 cells were subjected to SDS PAGE followed by western blot with 12398-1-AP (LBR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12398-1-AP (LBR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using LBR antibody 12398-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were stained with 0.2ug LBR antibody (12398-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LBR (IP:12398-1-AP, 3ug; Detection:12398-1-AP 1:500) with A375 cells lysate 3200ug.