H00003707-M01

antibody from Abnova Corporation

Targeting: ITPKB IP3-3KB, IP3KB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003707-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003707-M01, RRID:AB_509103

Product name

ITPKB monoclonal antibody (M01), clone 2F8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ITPKB.

Antigen sequence

PELLPQDQDKPFLRKACSPSNIPAVIITDMGTQED
GALEETQGSPRGNLPLRKLSSSSASSTGFSSSYED
SEEDISSDPERTLDPNSAFLHTLDQQKPR


Isotype

IgG

Antibody clone number

2F8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Human inositol 1,4,5-trisphosphate 3-kinase isoform B (IP3KB) is a nucleocytoplasmic shuttling protein specifically enriched at cortical actin filaments and at invaginations of the nuclear envelope.

Nalaskowski MM, Fliegert R, Ernst O, Brehm MA, Fanick W, Windhorst S, Lin H, Giehler S, Hein J, Lin YN, Mayr GW
The Journal of biological chemistry 2011 Feb 11;286(6):4500-10

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ITPKB monoclonal antibody (M01), clone 2F8 Western Blot analysis of ITPKB expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ITPKB expression in transfected 293T cell line by ITPKB monoclonal antibody (M01), clone 2F8.Lane 1: ITPKB transfected lysate(67.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ITPKB is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol