Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00003707-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003707-M01, RRID:AB_2280717
- Product name
- Mouse Monoclonal ITPKB Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ITPKB - inositol 1,4,5-trisphosphate 3-kinase B
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Human inositol 1,4,5-trisphosphate 3-kinase isoform B (IP3KB) is a nucleocytoplasmic shuttling protein specifically enriched at cortical actin filaments and at invaginations of the nuclear envelope.
Nalaskowski MM, Fliegert R, Ernst O, Brehm MA, Fanick W, Windhorst S, Lin H, Giehler S, Hein J, Lin YN, Mayr GW
The Journal of biological chemistry 2011 Feb 11;286(6):4500-10
The Journal of biological chemistry 2011 Feb 11;286(6):4500-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ITPKB Antibody (2F8) [H00003707-M01] - Analysis of ITPKB expression in transfected 293T cell line by ITPKB monoclonal antibody (M01), clone 2F8.Lane 1: ITPKB transfected lysate(67.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ITPKB Antibody (2F8) [H00003707-M01] - ITPKB monoclonal antibody (M01), clone 2F8 Analysis of ITPKB expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ITPKB Antibody (2F8) [H00003707-M01] - Detection limit for recombinant GST tagged ITPKB is 0.1 ng/ml as a capture antibody.