TA327344

antibody from OriGene

Targeting: H3-4 H3.4, H3/g, H3FT, H3t, HIST3H3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA327344

Provider

OriGene

Product name

DiMethyl-Histone H3-K4 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

DiMethyl-Histone H3-K4 Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

HIST3H3

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me2 antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of 293T cell using H3K4me2 antibody. Blue: DAPI for nuclear staining.

Validation comment

IF

Immunoprecipitation

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Validation comment

IP

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat lung tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded Rat liver using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded Rat kidney using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded Rat brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded Mouse lung using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded Mouse brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Validation comment

IHC

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Dot-blot analysis of all sorts of methylation peptides using H3K4me2 antibody.

Validation comment

DB