GTX54102

antibody from GeneTex

Targeting: H3-4 H3.4, H3/g, H3FT, H3t, HIST3H3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX54102

Provider

GeneTex

Product name

Histone H3K9me2 (di-methyl Lys9) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.

Zhang K, Wang J, Yang L, Yuan YC, Tong TR, Wu J, Yun X, Bonner M, Pangeni R, Liu Z, Yuchi T, Kim JY, Raz DJ
Molecular cancer 2018 Oct 22;17(1):153

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Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.

Eid W, Abdel-Rehim W
Biological chemistry 2016 Nov 1;397(11):1205-1213

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of HeLa lysate and H3 protein using Histone H3K9me2 (di-methyl Lys9) antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ICC/IF analysis of 293T cells using Histone H3K9me2 (di-methyl Lys9) antibody (red) and DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of rat liver tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human thyroid cancer tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of human rectal cancer tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of rat lung tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of rat testis tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of rat brain tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IHC-P analysis of mouse kidney tissue using Histone H3K9me2 (di-methyl Lys9) antibody at a dilution of 1:200.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ChIP assay was performed with 293 lysate and either Histone H3K9me2 (di-methyl Lys9) antibody or rabbit IgG. The precipitated DNA was detected by qPCR with primer set targeting to indicated genes. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Other assay

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Dot-blot analysis of all sorts of methylation peptides using Histone H3K9me2 (di-methyl Lys9) antibody.

Validation comment

Dot