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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 641011 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#641011, RRID:AB_2565889
- Product name
- PE/Cy7 anti-Histone H3-Phosphorylated (Ser28)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of human histone H3.
- Description
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Near infrared dye
- Antibody clone number
- HTA28
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HeLa cells (open histogram) and HeLa cells treated with Nocodazole for 24 hours (filled histogram), were fixed, permeabilized, and then stained with Histone H3-Phosphorylated (Ser28) (clone HTA28) PE/Cy7.
- Conjugate
- Near infrared dye
