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LearnPA5-96111
antibody from Invitrogen Antibodies
Targeting: H3-4
H3.4, H3/g, H3FT, H3t, HIST3H3
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-96111 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H3.1t Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: REIRRYQKST ELLIRKLPFQ RLMREIAQDF KTDLRFQSSA VMALQEACES YLVGLFEDTN LCVIHAKRVT IMPKDIQLAR RIRGERA; Positive Samples: Jurkat, A-431, Mouse liver, Mouse testis, Rat kidney; Cellular Location: Chromosome, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.17 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Histone H3.1t in extracts of various cell lines using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Histone H3.1t in extracts of various cell lines using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Histone H3.1t was performed in U2OS cells using Histone H3.1t Polyclonal Antibody (Product # PA5-96111).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded human appendix using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Histone H3.1t in paraffin-embedded mouse testis using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of Histone H3.1t in HeLa cell line using Histone H3.1t Polyclonal Antibody (Product # PA5-96111) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
