PA5-110611

antibody from Invitrogen Antibodies

Targeting: H3-4 H3.4, H3/g, H3FT, H3t, HIST3H3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110611

Provider

Invitrogen Antibodies

Product name

H3R8me1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.45 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of H3R8me1 in HeLa, H3 protein. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of H3R8me1 in 293T cells. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:100, followed by DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of H3R8me1 in paraffin-embedded human breast cancer tissue. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of H3R8me1 in paraffin-embedded mouse testis tissue. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of H3R8me1 in paraffin-embedded rat heart tissue. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Dot blot analysis of H3R8me1 in methylated peptides. Samples were incubated in polyclonal H3R8me1 antibody (Product # PA5-110611) using a dilution of 1:1000.