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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [45]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 07-449 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#07-449, RRID:AB_310624
- Product name
- Anti-trimethyl-Histone H3 (Lys27) Antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated, synthetic 2X-branched peptide containing the sequence AR(me3K)SAP in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µg
- Storage
- Stable for 1 year at -20°C from date of receipt.
Submitted references PRC2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors.
Hira-dependent histone H3.3 deposition facilitates PRC2 recruitment at developmental loci in ES cells.
Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas.
Macro histone variants are critical for the differentiation of human pluripotent cells.
XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells.
Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation.
The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
E-cadherin promotes incorporation of mouse epiblast stem cells into normal development.
Enhancer of zeste homolog 2 is overexpressed and contributes to epigenetic inactivation of p21 and phosphatase and tensin homolog in B-cell acute lymphoblastic leukemia.
Heterodimeric JAK-STAT activation as a mechanism of persistence to JAK2 inhibitor therapy.
Development of a novel approach, the epigenome-based outlier approach, to identify tumor-suppressor genes silenced by aberrant DNA methylation.
Asymmetrically modified nucleosomes.
Intrinsic properties of Tcf1 and Tcf4 splice variants determine cell-type-specific Wnt/β-catenin target gene expression.
Global identification of MLL2-targeted loci reveals MLL2's role in diverse signaling pathways.
A proteasome inhibitor-stimulated Nrf1 protein-dependent compensatory increase in proteasome subunit gene expression reduces polycomb group protein level.
Experience-dependent expression of NPAS4 regulates plasticity in adult visual cortex.
Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains.
The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression.
Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes.
The AT-hook motif-containing protein AHL22 regulates flowering initiation by modifying FLOWERING LOCUS T chromatin in Arabidopsis.
Profiles of epigenetic histone post-translational modifications at type 1 diabetes susceptible genes.
Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes.
Chromatin-modifying enzymes as modulators of reprogramming.
Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins.
Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
A functional role for the histone demethylase UTX in normal and malignant hematopoietic cells.
Sequential ChIP-bisulfite sequencing enables direct genome-scale investigation of chromatin and DNA methylation cross-talk.
Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA.
Chromatin state signatures associated with tissue-specific gene expression and enhancer activity in the embryonic limb.
Silencing of Wnt5a during colon cancer metastasis involves histone modifications.
CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.
Hypermethylated in cancer 1 (HIC1), a tumor suppressor gene epigenetically deregulated in hyperparathyroid tumors by histone H3 lysine modification.
Chromatin measurements reveal contributions of synthesis and decay to steady-state mRNA levels.
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis.
Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells.
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Postnatal development- and age-related changes in DNA-methylation patterns in the human genome.
IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics.
Ezh2 augments leukemogenicity by reinforcing differentiation blockage in acute myeloid leukemia.
A protective role for human IL-10-expressing CD4+ T cells in colitis.
Concerted epigenetic signatures inheritance at PcG targets through replication.
Heterochromatin formation in the mouse embryo requires critical residues of the histone variant H3.3.
Functional anatomy of polycomb and trithorax chromatin landscapes in Drosophila embryos.
Epigenetic analysis reveals a euchromatic configuration in the FMR1 unmethylated full mutations.
Lee W, Teckie S, Wiesner T, Ran L, Prieto Granada CN, Lin M, Zhu S, Cao Z, Liang Y, Sboner A, Tap WD, Fletcher JA, Huberman KH, Qin LX, Viale A, Singer S, Zheng D, Berger MF, Chen Y, Antonescu CR, Chi P
Nature genetics 2014 Nov;46(11):1227-32
Nature genetics 2014 Nov;46(11):1227-32
Hira-dependent histone H3.3 deposition facilitates PRC2 recruitment at developmental loci in ES cells.
Banaszynski LA, Wen D, Dewell S, Whitcomb SJ, Lin M, Diaz N, Elsässer SJ, Chapgier A, Goldberg AD, Canaani E, Rafii S, Zheng D, Allis CD
Cell 2013 Sep 26;155(1):107-20
Cell 2013 Sep 26;155(1):107-20
Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
Culver-Cochran AE, Chadwick BP
BMC genomics 2013 Oct 29;14:740
BMC genomics 2013 Oct 29;14:740
Reduced H3K27me3 and DNA hypomethylation are major drivers of gene expression in K27M mutant pediatric high-grade gliomas.
Bender S, Tang Y, Lindroth AM, Hovestadt V, Jones DT, Kool M, Zapatka M, Northcott PA, Sturm D, Wang W, Radlwimmer B, Højfeldt JW, Truffaux N, Castel D, Schubert S, Ryzhova M, Seker-Cin H, Gronych J, Johann PD, Stark S, Meyer J, Milde T, Schuhmann M, Ebinger M, Monoranu CM, Ponnuswami A, Chen S, Jones C, Witt O, Collins VP, von Deimling A, Jabado N, Puget S, Grill J, Helin K, Korshunov A, Lichter P, Monje M, Plass C, Cho YJ, Pfister SM
Cancer cell 2013 Nov 11;24(5):660-72
Cancer cell 2013 Nov 11;24(5):660-72
Macro histone variants are critical for the differentiation of human pluripotent cells.
Barrero MJ, Sese B, Martí M, Izpisua Belmonte JC
The Journal of biological chemistry 2013 May 31;288(22):16110-6
The Journal of biological chemistry 2013 May 31;288(22):16110-6
XACT, a long noncoding transcript coating the active X chromosome in human pluripotent cells.
Vallot C, Huret C, Lesecque Y, Resch A, Oudrhiri N, Bennaceur-Griscelli A, Duret L, Rougeulle C
Nature genetics 2013 Mar;45(3):239-41
Nature genetics 2013 Mar;45(3):239-41
Opposing roles of STAT4 and Dnmt3a in Th1 gene regulation.
Pham D, Yu Q, Walline CC, Muthukrishnan R, Blum JS, Kaplan MH
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):902-11
Journal of immunology (Baltimore, Md. : 1950) 2013 Jul 15;191(2):902-11
The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.
Vandamme J, Lettier G, Sidoli S, Di Schiavi E, Nørregaard Jensen O, Salcini AE
PLoS genetics 2012;8(5):e1002647
PLoS genetics 2012;8(5):e1002647
E-cadherin promotes incorporation of mouse epiblast stem cells into normal development.
Ohtsuka S, Nishikawa-Torikai S, Niwa H
PloS one 2012;7(9):e45220
PloS one 2012;7(9):e45220
Enhancer of zeste homolog 2 is overexpressed and contributes to epigenetic inactivation of p21 and phosphatase and tensin homolog in B-cell acute lymphoblastic leukemia.
Chen J, Li J, Han Q, Sun Z, Wang J, Wang S, Zhao RC
Experimental biology and medicine (Maywood, N.J.) 2012 Sep;237(9):1110-6
Experimental biology and medicine (Maywood, N.J.) 2012 Sep;237(9):1110-6
Heterodimeric JAK-STAT activation as a mechanism of persistence to JAK2 inhibitor therapy.
Koppikar P, Bhagwat N, Kilpivaara O, Manshouri T, Adli M, Hricik T, Liu F, Saunders LM, Mullally A, Abdel-Wahab O, Leung L, Weinstein A, Marubayashi S, Goel A, Gönen M, Estrov Z, Ebert BL, Chiosis G, Nimer SD, Bernstein BE, Verstovsek S, Levine RL
Nature 2012 Sep 6;489(7414):155-9
Nature 2012 Sep 6;489(7414):155-9
Development of a novel approach, the epigenome-based outlier approach, to identify tumor-suppressor genes silenced by aberrant DNA methylation.
Kikuyama M, Takeshima H, Kinoshita T, Okochi-Takada E, Wakabayashi M, Akashi-Tanaka S, Ogawa T, Seto Y, Ushijima T
Cancer letters 2012 Sep 28;322(2):204-12
Cancer letters 2012 Sep 28;322(2):204-12
Asymmetrically modified nucleosomes.
Voigt P, LeRoy G, Drury WJ 3rd, Zee BM, Son J, Beck DB, Young NL, Garcia BA, Reinberg D
Cell 2012 Sep 28;151(1):181-93
Cell 2012 Sep 28;151(1):181-93
Intrinsic properties of Tcf1 and Tcf4 splice variants determine cell-type-specific Wnt/β-catenin target gene expression.
Wallmen B, Schrempp M, Hecht A
Nucleic acids research 2012 Oct;40(19):9455-69
Nucleic acids research 2012 Oct;40(19):9455-69
Global identification of MLL2-targeted loci reveals MLL2's role in diverse signaling pathways.
Guo C, Chang CC, Wortham M, Chen LH, Kernagis DN, Qin X, Cho YW, Chi JT, Grant GA, McLendon RE, Yan H, Ge K, Papadopoulos N, Bigner DD, He Y
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17603-8
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17603-8
A proteasome inhibitor-stimulated Nrf1 protein-dependent compensatory increase in proteasome subunit gene expression reduces polycomb group protein level.
Balasubramanian S, Kanade S, Han B, Eckert RL
The Journal of biological chemistry 2012 Oct 19;287(43):36179-89
The Journal of biological chemistry 2012 Oct 19;287(43):36179-89
Experience-dependent expression of NPAS4 regulates plasticity in adult visual cortex.
Maya-Vetencourt JF, Tiraboschi E, Greco D, Restani L, Cerri C, Auvinen P, Maffei L, Castrén E
The Journal of physiology 2012 Oct 1;590(Pt 19):4777-87
The Journal of physiology 2012 Oct 1;590(Pt 19):4777-87
Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains.
Van Bortle K, Ramos E, Takenaka N, Yang J, Wahi JE, Corces VG
Genome research 2012 Nov;22(11):2176-87
Genome research 2012 Nov;22(11):2176-87
The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression.
Babbio F, Pistore C, Curti L, Castiglioni I, Kunderfranco P, Brino L, Oudet P, Seiler R, Thalman GN, Roggero E, Sarti M, Pinton S, Mello-Grand M, Chiorino G, Catapano CV, Carbone GM, Bonapace IM
Oncogene 2012 Nov 15;31(46):4878-87
Oncogene 2012 Nov 15;31(46):4878-87
Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes.
Wells MB, Snyder MJ, Custer LM, Csankovszki G
Molecular and cellular biology 2012 May;32(9):1710-9
Molecular and cellular biology 2012 May;32(9):1710-9
The AT-hook motif-containing protein AHL22 regulates flowering initiation by modifying FLOWERING LOCUS T chromatin in Arabidopsis.
Yun J, Kim YS, Jung JH, Seo PJ, Park CM
The Journal of biological chemistry 2012 May 4;287(19):15307-16
The Journal of biological chemistry 2012 May 4;287(19):15307-16
Profiles of epigenetic histone post-translational modifications at type 1 diabetes susceptible genes.
Miao F, Chen Z, Zhang L, Liu Z, Wu X, Yuan YC, Natarajan R
The Journal of biological chemistry 2012 May 11;287(20):16335-45
The Journal of biological chemistry 2012 May 11;287(20):16335-45
Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes.
Rodríguez-Ubreva J, Ciudad L, Gómez-Cabrero D, Parra M, Bussmann LH, di Tullio A, Kallin EM, Tegnér J, Graf T, Ballestar E
Nucleic acids research 2012 Mar;40(5):1954-68
Nucleic acids research 2012 Mar;40(5):1954-68
Chromatin-modifying enzymes as modulators of reprogramming.
Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, Daley GQ
Nature 2012 Mar 4;483(7391):598-602
Nature 2012 Mar 4;483(7391):598-602
Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins.
Boulay G, Dubuissez M, Van Rechem C, Forget A, Helin K, Ayrault O, Leprince D
The Journal of biological chemistry 2012 Mar 23;287(13):10509-24
The Journal of biological chemistry 2012 Mar 23;287(13):10509-24
Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
Sustáčková G, Legartová S, Kozubek S, Stixová L, Pacherník J, Bártová E
Stem cells and development 2012 Mar 20;21(5):710-20
Stem cells and development 2012 Mar 20;21(5):710-20
A functional role for the histone demethylase UTX in normal and malignant hematopoietic cells.
Liu J, Mercher T, Scholl C, Brumme K, Gilliland DG, Zhu N
Experimental hematology 2012 Jun;40(6):487-98.e3
Experimental hematology 2012 Jun;40(6):487-98.e3
Sequential ChIP-bisulfite sequencing enables direct genome-scale investigation of chromatin and DNA methylation cross-talk.
Brinkman AB, Gu H, Bartels SJ, Zhang Y, Matarese F, Simmer F, Marks H, Bock C, Gnirke A, Meissner A, Stunnenberg HG
Genome research 2012 Jun;22(6):1128-38
Genome research 2012 Jun;22(6):1128-38
Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA.
Statham AL, Robinson MD, Song JZ, Coolen MW, Stirzaker C, Clark SJ
Genome research 2012 Jun;22(6):1120-7
Genome research 2012 Jun;22(6):1120-7
Chromatin state signatures associated with tissue-specific gene expression and enhancer activity in the embryonic limb.
Cotney J, Leng J, Oh S, DeMare LE, Reilly SK, Gerstein MB, Noonan JP
Genome research 2012 Jun;22(6):1069-80
Genome research 2012 Jun;22(6):1069-80
Silencing of Wnt5a during colon cancer metastasis involves histone modifications.
Li Q, Chen H
Epigenetics : official journal of the DNA Methylation Society 2012 Jun 1;7(6):551-8
Epigenetics : official journal of the DNA Methylation Society 2012 Jun 1;7(6):551-8
CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.
Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5655-64
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5655-64
Hypermethylated in cancer 1 (HIC1), a tumor suppressor gene epigenetically deregulated in hyperparathyroid tumors by histone H3 lysine modification.
Svedlund J, Koskinen Edblom S, Marquez VE, Åkerström G, Björklund P, Westin G
The Journal of clinical endocrinology and metabolism 2012 Jul;97(7):E1307-15
The Journal of clinical endocrinology and metabolism 2012 Jul;97(7):E1307-15
Chromatin measurements reveal contributions of synthesis and decay to steady-state mRNA levels.
Tippmann SC, Ivanek R, Gaidatzis D, Schöler A, Hoerner L, van Nimwegen E, Stadler PF, Stadler MB, Schübeler D
Molecular systems biology 2012 Jul 17;8:593
Molecular systems biology 2012 Jul 17;8:593
Epigenetic repression of cardiac progenitor gene expression by Ezh2 is required for postnatal cardiac homeostasis.
Delgado-Olguín P, Huang Y, Li X, Christodoulou D, Seidman CE, Seidman JG, Tarakhovsky A, Bruneau BG
Nature genetics 2012 Jan 22;44(3):343-7
Nature genetics 2012 Jan 22;44(3):343-7
Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells.
Neri F, Zippo A, Krepelova A, Cherubini A, Rocchigiani M, Oliviero S
Molecular and cellular biology 2012 Feb;32(4):840-51
Molecular and cellular biology 2012 Feb;32(4):840-51
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB
Nature 2012 Feb 15;483(7390):474-8
Nature 2012 Feb 15;483(7390):474-8
Postnatal development- and age-related changes in DNA-methylation patterns in the human genome.
Salpea P, Russanova VR, Hirai TH, Sourlingas TG, Sekeri-Pataryas KE, Romero R, Epstein J, Howard BH
Nucleic acids research 2012 Aug;40(14):6477-94
Nucleic acids research 2012 Aug;40(14):6477-94
IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics.
Sasaki M, Knobbe CB, Munger JC, Lind EF, Brenner D, Brüstle A, Harris IS, Holmes R, Wakeham A, Haight J, You-Ten A, Li WY, Schalm S, Su SM, Virtanen C, Reifenberger G, Ohashi PS, Barber DL, Figueroa ME, Melnick A, Zúñiga-Pflücker JC, Mak TW
Nature 2012 Aug 30;488(7413):656-9
Nature 2012 Aug 30;488(7413):656-9
Ezh2 augments leukemogenicity by reinforcing differentiation blockage in acute myeloid leukemia.
Tanaka S, Miyagi S, Sashida G, Chiba T, Yuan J, Mochizuki-Kashio M, Suzuki Y, Sugano S, Nakaseko C, Yokote K, Koseki H, Iwama A
Blood 2012 Aug 2;120(5):1107-17
Blood 2012 Aug 2;120(5):1107-17
A protective role for human IL-10-expressing CD4+ T cells in colitis.
Ranatunga DC, Ramakrishnan A, Uprety P, Wang F, Zhang H, Margolick JB, Brayton C, Bream JH
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1243-52
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1243-52
Concerted epigenetic signatures inheritance at PcG targets through replication.
Lanzuolo C, Lo Sardo F, Orlando V
Cell cycle (Georgetown, Tex.) 2012 Apr 1;11(7):1296-300
Cell cycle (Georgetown, Tex.) 2012 Apr 1;11(7):1296-300
Heterochromatin formation in the mouse embryo requires critical residues of the histone variant H3.3.
Santenard A, Ziegler-Birling C, Koch M, Tora L, Bannister AJ, Torres-Padilla ME
Nature cell biology 2010 Sep;12(9):853-62
Nature cell biology 2010 Sep;12(9):853-62
Functional anatomy of polycomb and trithorax chromatin landscapes in Drosophila embryos.
Schuettengruber B, Ganapathi M, Leblanc B, Portoso M, Jaschek R, Tolhuis B, van Lohuizen M, Tanay A, Cavalli G
PLoS biology 2009 Jan 13;7(1):e13
PLoS biology 2009 Jan 13;7(1):e13
Epigenetic analysis reveals a euchromatic configuration in the FMR1 unmethylated full mutations.
Tabolacci E, Moscato U, Zalfa F, Bagni C, Chiurazzi P, Neri G
European journal of human genetics : EJHG 2008 Dec;16(12):1487-98
European journal of human genetics : EJHG 2008 Dec;16(12):1487-98
No comments: Submit comment
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- WB: 0.1µg/ml detected trimethylated Histone H3 in 20 micrograms of acid extracted proteins from HeLa cells (Lane 2), but did not detect unmethylated recombinant Histone H3 (14-411) (Lane 1).
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- ICC: Mouse embryonic fibroblasts were stained with Anti-trimethyl-Histone H3 (Lys27).
- Validation comment
- ICC
