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- Antigen structure
- References [11]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 05-806 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#05-806, RRID:AB_310016
- Product name
- Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
- Antibody type
- Monoclonal
- Antigen
- A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H10
- Vial size
- 100 µg
- Storage
- Stable for 1 year at 2 to 8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Submitted references TET2 promoter methylation in low-grade diffuse gliomas lacking IDH1/2 mutations.
Role for hACF1 in the G2/M damage checkpoint.
Familiar taste induces higher dendritic levels of activity-regulated cytoskeleton-associated protein in the insular cortex than a novel one.
An integrated chemical biology approach identifies specific vulnerability of Ewing's sarcoma to combined inhibition of Aurora kinases A and B.
Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres.
The mef/elf4 transcription factor fine tunes the DNA damage response.
Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
Steel factor controls midline cell death of primordial germ cells and is essential for their normal proliferation and migration.
Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells.
Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase.
Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors.
Kim YH, Pierscianek D, Mittelbronn M, Vital A, Mariani L, Hasselblatt M, Ohgaki H
Journal of clinical pathology 2011 Oct;64(10):850-2
Journal of clinical pathology 2011 Oct;64(10):850-2
Role for hACF1 in the G2/M damage checkpoint.
Sánchez-Molina S, Mortusewicz O, Bieber B, Auer S, Eckey M, Leonhardt H, Friedl AA, Becker PB
Nucleic acids research 2011 Oct;39(19):8445-56
Nucleic acids research 2011 Oct;39(19):8445-56
Familiar taste induces higher dendritic levels of activity-regulated cytoskeleton-associated protein in the insular cortex than a novel one.
Morin JP, Quiroz C, Mendoza-Viveros L, Ramirez-Amaya V, Bermudez-Rattoni F
Learning & memory (Cold Spring Harbor, N.Y.) 2011 Oct;18(10):610-6
Learning & memory (Cold Spring Harbor, N.Y.) 2011 Oct;18(10):610-6
An integrated chemical biology approach identifies specific vulnerability of Ewing's sarcoma to combined inhibition of Aurora kinases A and B.
Winter GE, Rix U, Lissat A, Stukalov A, Müllner MK, Bennett KL, Colinge J, Nijman SM, Kubicek S, Kovar H, Kontny U, Superti-Furga G
Molecular cancer therapeutics 2011 Oct;10(10):1846-56
Molecular cancer therapeutics 2011 Oct;10(10):1846-56
Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres.
Dheur S, Saupe SJ, Genier S, Vazquez S, Javerzat JP
Molecular and cellular biology 2011 Mar;31(5):1088-97
Molecular and cellular biology 2011 Mar;31(5):1088-97
The mef/elf4 transcription factor fine tunes the DNA damage response.
Sashida G, Bae N, Di Giandomenico S, Asai T, Gurvich N, Bazzoli E, Liu Y, Huang G, Zhao X, Menendez S, Nimer SD
Cancer research 2011 Jul 15;71(14):4857-65
Cancer research 2011 Jul 15;71(14):4857-65
Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
Reed JR, Leon RP, Hall MK, Schwertfeger KL
Breast cancer research : BCR 2009;11(2):R21
Breast cancer research : BCR 2009;11(2):R21
Steel factor controls midline cell death of primordial germ cells and is essential for their normal proliferation and migration.
Runyan C, Schaible K, Molyneaux K, Wang Z, Levin L, Wylie C
Development (Cambridge, England) 2006 Dec;133(24):4861-9
Development (Cambridge, England) 2006 Dec;133(24):4861-9
Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells.
Ajiro K, Yoda K, Utsumi K, Nishikawa Y
The Journal of biological chemistry 1996 May 31;271(22):13197-201
The Journal of biological chemistry 1996 May 31;271(22):13197-201
Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase.
Chadee DN, Taylor WR, Hurta RA, Allis CD, Wright JA, Davie JR
The Journal of biological chemistry 1995 Aug 25;270(34):20098-105
The Journal of biological chemistry 1995 Aug 25;270(34):20098-105
Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors.
Mahadevan LC, Willis AC, Barratt MJ
Cell 1991 May 31;65(5):775-83
Cell 1991 May 31;65(5):775-83
No comments: Submit comment
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- WB: Acid extract from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells, along with recombinant Histone H3 (Lane 3) were probed with anti-phospho-Histone H3 (Ser10) (0.1_g/ml).
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- IF: positive chromosome immunostaining for mitotic A431 and HeLa cells fixed with 95% ethanol and 5% acetic acid and permeabilized with 0.1% Triton X-100
- Validation comment
- IF
