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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-14117 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p73 Monoclonal Antibody (ER-15)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-14117 targets p73alpha/beta in IP and WB applications and shows reactivity with Human and Non-human primate samples.
- Antibody clone number
- ER-15
- Concentration
- 0.2 mg/mL
Submitted references Analysis of the Expression Pattern of Cajal-Retzius Cell Markers in the Xenopus laevis Forebrain.
Early dorsomedial tissue interactions regulate gyrification of distal neocortex.
SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity.
E3 ubiquitin ligase TRIM32 negatively regulates tumor suppressor p53 to promote tumorigenesis.
The differential effects of wild-type and mutated K-Ras on MST2 signaling are determined by K-Ras activation kinetics.
TAp73β-mediated suppression of cell migration requires p57Kip2 control of actin cytoskeleton dynamics.
IkappaB kinase beta promotes cell survival by antagonizing p53 functions through DeltaNp73alpha phosphorylation and stabilization.
Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway.
Sp1-like sequences mediate human caspase-3 promoter activation by p73 and cisplatin.
Resistance of mitochondrial p53 to dominant inhibition.
PML, YAP, and p73 are components of a proapoptotic autoregulatory feedback loop.
Zic deficiency in the cortical marginal zone and meninges results in cortical lamination defects resembling those in type II lissencephaly.
Differential regulation of vitamin D receptor (VDR) by the p53 Family: p73-dependent induction of VDR upon DNA damage.
p73 expression in medulloblastoma: TAp73/DeltaNp73 transcript detection and possible association of p73alpha/DeltaNp73 immunoreactivity with survival.
Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes.
p53 dependent and independent sensitivity to oxaliplatin of colon cancer cells.
S100A2 gene is a direct transcriptional target of p53 homologues during keratinocyte differentiation.
A novel response element confers p63- and p73-specific activation of the WNT4 promoter.
Over-expression of p73beta results in apoptotic death of post-mitotic hNT neurons.
Massive loss of Cajal-Retzius cells does not disrupt neocortical layer order.
MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells.
Full-length p73alpha represses drug-induced apoptosis in small cell lung carcinoma cells.
deltaEF1 repressor controls selectively p53 family members during differentiation.
The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage.
p63-specific activation of the BPAG-1e promoter.
Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53.
New antibodies recognizing p73: comparison with commercial antibodies.
Developmental roles of p73 in Cajal-Retzius cells and cortical patterning.
ASPP1 and ASPP2: common activators of p53 family members.
p73 tumor-suppressor activity is impaired in human thyroid cancer.
p73 Overexpression and angiogenesis in human colorectal carcinoma.
Exclusion of c-Abl from the nucleus restrains the p73 tumor suppression function.
p53 polymorphism influences response in cancer chemotherapy via modulation of p73-dependent apoptosis.
Expression of p73 and Reelin in the developing human cortex.
Identification and characterization of HIPK2 interacting with p73 and modulating functions of the p53 family in vivo.
Physical interaction of p73 with c-Myc and MM1, a c-Myc-binding protein, and modulation of the p73 function.
Jiménez S, Moreno N
Brain, behavior and evolution 2022;96(4-6):263-282
Brain, behavior and evolution 2022;96(4-6):263-282
Early dorsomedial tissue interactions regulate gyrification of distal neocortex.
Chizhikov VV, Iskusnykh IY, Steshina EY, Fattakhov N, Lindgren AG, Shetty AS, Roy A, Tole S, Millen KJ
Nature communications 2019 Nov 15;10(1):5192
Nature communications 2019 Nov 15;10(1):5192
SIRT2-mediated inactivation of p73 is required for glioblastoma tumorigenicity.
Funato K, Hayashi T, Echizen K, Negishi L, Shimizu N, Koyama-Nasu R, Nasu-Nishimura Y, Morishita Y, Tabar V, Todo T, Ino Y, Mukasa A, Saito N, Akiyama T
EMBO reports 2018 Nov;19(11)
EMBO reports 2018 Nov;19(11)
E3 ubiquitin ligase TRIM32 negatively regulates tumor suppressor p53 to promote tumorigenesis.
Liu J, Zhang C, Wang XL, Ly P, Belyi V, Xu-Monette ZY, Young KH, Hu W, Feng Z
Cell death and differentiation 2014 Nov;21(11):1792-804
Cell death and differentiation 2014 Nov;21(11):1792-804
The differential effects of wild-type and mutated K-Ras on MST2 signaling are determined by K-Ras activation kinetics.
Romano D, Maccario H, Doherty C, Quinn NP, Kolch W, Matallanas D
Molecular and cellular biology 2013 May;33(9):1859-68
Molecular and cellular biology 2013 May;33(9):1859-68
TAp73β-mediated suppression of cell migration requires p57Kip2 control of actin cytoskeleton dynamics.
Rodhe J, Kavanagh E, Joseph B
Oncotarget 2013 Feb;4(2):289-97
Oncotarget 2013 Feb;4(2):289-97
IkappaB kinase beta promotes cell survival by antagonizing p53 functions through DeltaNp73alpha phosphorylation and stabilization.
Accardi R, Scalise M, Gheit T, Hussain I, Yue J, Carreira C, Collino A, Indiveri C, Gissmann L, Sylla BS, Tommasino M
Molecular and cellular biology 2011 Jun;31(11):2210-26
Molecular and cellular biology 2011 Jun;31(11):2210-26
Isoform-specific p73 knockout mice reveal a novel role for delta Np73 in the DNA damage response pathway.
Wilhelm MT, Rufini A, Wetzel MK, Tsuchihara K, Inoue S, Tomasini R, Itie-Youten A, Wakeham A, Arsenian-Henriksson M, Melino G, Kaplan DR, Miller FD, Mak TW
Genes & development 2010 Mar 15;24(6):549-60
Genes & development 2010 Mar 15;24(6):549-60
Sp1-like sequences mediate human caspase-3 promoter activation by p73 and cisplatin.
Sudhakar C, Jain N, Swarup G
The FEBS journal 2008 May;275(9):2200-13
The FEBS journal 2008 May;275(9):2200-13
Resistance of mitochondrial p53 to dominant inhibition.
Heyne K, Schmitt K, Mueller D, Armbruester V, Mestres P, Roemer K
Molecular cancer 2008 Jun 12;7:54
Molecular cancer 2008 Jun 12;7:54
PML, YAP, and p73 are components of a proapoptotic autoregulatory feedback loop.
Lapi E, Di Agostino S, Donzelli S, Gal H, Domany E, Rechavi G, Pandolfi PP, Givol D, Strano S, Lu X, Blandino G
Molecular cell 2008 Dec 26;32(6):803-14
Molecular cell 2008 Dec 26;32(6):803-14
Zic deficiency in the cortical marginal zone and meninges results in cortical lamination defects resembling those in type II lissencephaly.
Inoue T, Ogawa M, Mikoshiba K, Aruga J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 30;28(18):4712-25
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Apr 30;28(18):4712-25
Differential regulation of vitamin D receptor (VDR) by the p53 Family: p73-dependent induction of VDR upon DNA damage.
Kommagani R, Payal V, Kadakia MP
The Journal of biological chemistry 2007 Oct 12;282(41):29847-54
The Journal of biological chemistry 2007 Oct 12;282(41):29847-54
p73 expression in medulloblastoma: TAp73/DeltaNp73 transcript detection and possible association of p73alpha/DeltaNp73 immunoreactivity with survival.
Zitterbart K, Zavrelova I, Kadlecova J, Spesna R, Kratochvilova A, Pavelka Z, Sterba J
Acta neuropathologica 2007 Dec;114(6):641-50
Acta neuropathologica 2007 Dec;114(6):641-50
Bi-compartmental communication contributes to the opposite proliferative behavior of Notch1-deficient hair follicle and epidermal keratinocytes.
Lee J, Basak JM, Demehri S, Kopan R
Development (Cambridge, England) 2007 Aug;134(15):2795-806
Development (Cambridge, England) 2007 Aug;134(15):2795-806
p53 dependent and independent sensitivity to oxaliplatin of colon cancer cells.
Toscano F, Parmentier B, Fajoui ZE, Estornes Y, Chayvialle JA, Saurin JC, Abello J
Biochemical pharmacology 2007 Aug 1;74(3):392-406
Biochemical pharmacology 2007 Aug 1;74(3):392-406
S100A2 gene is a direct transcriptional target of p53 homologues during keratinocyte differentiation.
Lapi E, Iovino A, Fontemaggi G, Soliera AR, Iacovelli S, Sacchi A, Rechavi G, Givol D, Blandino G, Strano S
Oncogene 2006 Jun 22;25(26):3628-37
Oncogene 2006 Jun 22;25(26):3628-37
A novel response element confers p63- and p73-specific activation of the WNT4 promoter.
Osada M, Park HL, Nagakawa Y, Begum S, Yamashita K, Wu G, Kim MS, Trink B, Sidransky D
Biochemical and biophysical research communications 2006 Jan 27;339(4):1120-8
Biochemical and biophysical research communications 2006 Jan 27;339(4):1120-8
Over-expression of p73beta results in apoptotic death of post-mitotic hNT neurons.
Jiang Y, Lo W, Akhmametyeva EM, Chang LS
Journal of the neurological sciences 2006 Jan 15;240(1-2):1-6
Journal of the neurological sciences 2006 Jan 15;240(1-2):1-6
Massive loss of Cajal-Retzius cells does not disrupt neocortical layer order.
Yoshida M, Assimacopoulos S, Jones KR, Grove EA
Development (Cambridge, England) 2006 Feb;133(3):537-45
Development (Cambridge, England) 2006 Feb;133(3):537-45
MDM2 is required for suppression of apoptosis by activated Akt1 in salivary acinar cells.
Limesand KH, Schwertfeger KL, Anderson SM
Molecular and cellular biology 2006 Dec;26(23):8840-56
Molecular and cellular biology 2006 Dec;26(23):8840-56
Full-length p73alpha represses drug-induced apoptosis in small cell lung carcinoma cells.
Nyman U, Sobczak-Pluta A, Vlachos P, Perlmann T, Zhivotovsky B, Joseph B
The Journal of biological chemistry 2005 Oct 7;280(40):34159-69
The Journal of biological chemistry 2005 Oct 7;280(40):34159-69
deltaEF1 repressor controls selectively p53 family members during differentiation.
Fontemaggi G, Gurtner A, Damalas A, Costanzo A, Higashi Y, Sacchi A, Strano S, Piaggio G, Blandino G
Oncogene 2005 Nov 10;24(49):7273-80
Oncogene 2005 Nov 10;24(49):7273-80
The transcriptional coactivator Yes-associated protein drives p73 gene-target specificity in response to DNA Damage.
Strano S, Monti O, Pediconi N, Baccarini A, Fontemaggi G, Lapi E, Mantovani F, Damalas A, Citro G, Sacchi A, Del Sal G, Levrero M, Blandino G
Molecular cell 2005 May 13;18(4):447-59
Molecular cell 2005 May 13;18(4):447-59
p63-specific activation of the BPAG-1e promoter.
Osada M, Nagakawa Y, Park HL, Yamashita K, Wu G, Kim MS, Fomenkov A, Trink B, Sidransky D
The Journal of investigative dermatology 2005 Jul;125(1):52-60
The Journal of investigative dermatology 2005 Jul;125(1):52-60
Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53.
Golubovskaya VM, Finch R, Cance WG
The Journal of biological chemistry 2005 Jul 1;280(26):25008-21
The Journal of biological chemistry 2005 Jul 1;280(26):25008-21
New antibodies recognizing p73: comparison with commercial antibodies.
Sayan AE, Paradisi A, Vojtesek B, Knight RA, Melino G, Candi E
Biochemical and biophysical research communications 2005 Apr 29;330(1):186-93
Biochemical and biophysical research communications 2005 Apr 29;330(1):186-93
Developmental roles of p73 in Cajal-Retzius cells and cortical patterning.
Meyer G, Cabrera Socorro A, Perez Garcia CG, Martinez Millan L, Walker N, Caput D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Nov 3;24(44):9878-87
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Nov 3;24(44):9878-87
ASPP1 and ASPP2: common activators of p53 family members.
Bergamaschi D, Samuels Y, Jin B, Duraisingham S, Crook T, Lu X
Molecular and cellular biology 2004 Feb;24(3):1341-50
Molecular and cellular biology 2004 Feb;24(3):1341-50
p73 tumor-suppressor activity is impaired in human thyroid cancer.
Frasca F, Vella V, Aloisi A, Mandarino A, Mazzon E, Vigneri R, Vigneri P
Cancer research 2003 Sep 15;63(18):5829-37
Cancer research 2003 Sep 15;63(18):5829-37
p73 Overexpression and angiogenesis in human colorectal carcinoma.
Guan M, Peng HX, Yu B, Lu Y
Japanese journal of clinical oncology 2003 May;33(5):215-20
Japanese journal of clinical oncology 2003 May;33(5):215-20
Exclusion of c-Abl from the nucleus restrains the p73 tumor suppression function.
Vella V, Zhu J, Frasca F, Li CY, Vigneri P, Vigneri R, Wang JY
The Journal of biological chemistry 2003 Jul 4;278(27):25151-7
The Journal of biological chemistry 2003 Jul 4;278(27):25151-7
p53 polymorphism influences response in cancer chemotherapy via modulation of p73-dependent apoptosis.
Bergamaschi D, Gasco M, Hiller L, Sullivan A, Syed N, Trigiante G, Yulug I, Merlano M, Numico G, Comino A, Attard M, Reelfs O, Gusterson B, Bell AK, Heath V, Tavassoli M, Farrell PJ, Smith P, Lu X, Crook T
Cancer cell 2003 Apr;3(4):387-402
Cancer cell 2003 Apr;3(4):387-402
Expression of p73 and Reelin in the developing human cortex.
Meyer G, Perez-Garcia CG, Abraham H, Caput D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2002 Jun 15;22(12):4973-86
The Journal of neuroscience : the official journal of the Society for Neuroscience 2002 Jun 15;22(12):4973-86
Identification and characterization of HIPK2 interacting with p73 and modulating functions of the p53 family in vivo.
Kim EJ, Park JS, Um SJ
The Journal of biological chemistry 2002 Aug 30;277(35):32020-8
The Journal of biological chemistry 2002 Aug 30;277(35):32020-8
Physical interaction of p73 with c-Myc and MM1, a c-Myc-binding protein, and modulation of the p73 function.
Watanabe K, Ozaki T, Nakagawa T, Miyazaki K, Takahashi M, Hosoda M, Hayashi S, Todo S, Nakagawara A
The Journal of biological chemistry 2002 Apr 26;277(17):15113-23
The Journal of biological chemistry 2002 Apr 26;277(17):15113-23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of MCF7 (Lane 1) and MCF7 treated with Doxorubicine (0.5 uM Doxorubicin for 24 hours) (Lane 2). The blot was probed with Anti-p73 alpha/beta Mouse Monoclonal Antibody (Product # MA5-14117, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 39 kDa and 44 kDa corresponding to p73 alpha/beta were observed across the cell lines and was enhanced upon treatment. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of p73 alpha/beta was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p73 alpha/beta Antibody (ER-15) Mouse Monoclonal Antibody (Product # MA5-14117) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
