12405-1-AP

antibody from Invitrogen Antibodies

Targeting: NUP133 FLJ10814

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12405-1-AP

Provider

Invitrogen Antibodies

Product name

NUP133 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LWAASLAEK YCDFDILVQM CEQTDNQSRL QRYMTQFADQ NFSDFLFRWY LEKGKRGKLL SQPISQHGQL ANFLQAHEHL SWLHEINSQE LEKAHATLLG LANMETRYFA KKKTLLGLSK LAALASDFSE DMLQEKIEEM AEQERFLLHQ ETLPEQLLAE KQLNLSAMPV LTAPQLIGLY ICEENRRANE YDFKKALDLL EYIDEEEDIN INDLKLEILC KALQRDNWSS SDGKDDPIEV SKDSIFVKIL QKLLKDGIQL SEYLPEVKDL LQADQLGSLK SNPYFEFVLK ANYEYYVQGQ I (857-1156 aa encoded by BC020107)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12405-1-AP (NUP133 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 12405-1-AP) (NUP133 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NUP133 (IP:12405-1-AP, 4ug; Detection:12405-1-AP 1:800) with HeLa cells lysate 880ug.