Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14931-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNPAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP ( GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody 14931-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using GNPAT antibody 14931-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.