Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-25142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERO1LB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.
Yu XX, Qiu WL, Yang L, Wang YC, He MY, Wang D, Zhang Y, Li LC, Zhang J, Wang Y, Xu CR
Cell research 2021 Aug;31(8):886-903
Cell research 2021 Aug;31(8):886-903
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an ERO1LB polyclonal antibody (Product # PA5-25142) in MDA-MB435 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with Thapsigargin (1uM for 24 hrs) (Lane 2), PC-3 (Lane 3), IM9 (Lane 4), PANC-1 (Lane 5) and AML193 (Lane 6). The blot was probed with Anti-ERO1LB Polyclonal Antibody (Product # PA5-25142, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 54 kDa band corresponding to ERO1LB was observed in the cell line tested and enhanced upon treatment in A549 cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded mouse testis tissue using an ERO1LB polyclonal antibody (Product # PA5-25142), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MDA-MB435 cells using an ERO1LB polyclonal antibody (Product # PA5-25142) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
