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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [5]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34693 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Galectin 8 Recombinant Rabbit Monoclonal Antibody (JB85-35)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Mouse testis and rat testis tissue lysates, Human liver tissue, human colon cancer tissue, human kidney tissue, HepG2, A431, PC-3M.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB85-35
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin 8 in mouse testis and rat testis tissue lysates. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Galectin 8 in mouse testis and rat testis tissue lysates. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Galectin 8 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Galectin 8 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Galectin 8 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human liver tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human kidney tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Galectin 8 in paraffin-embedded human colon cancer tissue. Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Galectin 8 in PC-3M cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Galectin 8 monoclonal antibody (Product # MA5-34693) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
