BSM-54301R

antibody from Invitrogen Antibodies

Targeting: LGALS8 PCTA-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

BSM-54301R

Provider

Invitrogen Antibodies

Product name

Galectin 8 Monoclonal Antibody (1A4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine bone osteonectin (SPARC). The monoclonal antibody was harvested from ascitic fluid.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

1A4

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1: Mouse Testis; Lane 2: Rat Testis; Probed with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF(ICC) staining with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF(ICC) staining with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PC-3M cells with Galectin 8 (1A4) Monoclonal Antibody (bsm-54301R) at a 1:100 dilution (red) compared with an unlabeled control (cells with out incubation with primary antibody; black).