Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF1305 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-8 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O00214
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human trophoblast requires galectin-3 for cell migration and invasion.
Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells.
Galectin-8-mediated selective autophagy protects against seeded tau aggregation.
A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells.
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
Galectin-8 promotes cytoskeletal rearrangement in trabecular meshwork cells through activation of Rho signaling.
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Bojić-Trbojević Ž, Jovanović Krivokuća M, Vilotić A, Kolundžić N, Stefanoska I, Zetterberg F, Nilsson UJ, Leffler H, Vićovac L
Scientific reports 2019 Feb 14;9(1):2136
Scientific reports 2019 Feb 14;9(1):2136
Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells.
Lerner TR, Queval CJ, Fearns A, Repnik U, Griffiths G, Gutierrez MG
BMC biology 2018 Jan 4;16(1):1
BMC biology 2018 Jan 4;16(1):1
Galectin-8-mediated selective autophagy protects against seeded tau aggregation.
Falcon B, Noad J, McMahon H, Randow F, Goedert M
The Journal of biological chemistry 2018 Feb 16;293(7):2438-2451
The Journal of biological chemistry 2018 Feb 16;293(7):2438-2451
A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
Schnettger L, Rodgers A, Repnik U, Lai RP, Pei G, Verdoes M, Wilkinson RJ, Young DB, Gutierrez MG
Cell host & microbe 2017 May 10;21(5):619-628.e5
Cell host & microbe 2017 May 10;21(5):619-628.e5
K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells.
Clough B, Wright JD, Pereira PM, Hirst EM, Johnston AC, Henriques R, Frickel EM
PLoS pathogens 2016 Nov;12(11):e1006027
PLoS pathogens 2016 Nov;12(11):e1006027
Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases.
Meunier E, Dick MS, Dreier RF, Schürmann N, Kenzelmann Broz D, Warming S, Roose-Girma M, Bumann D, Kayagaki N, Takeda K, Yamamoto M, Broz P
Nature 2014 May 15;509(7500):366-70
Nature 2014 May 15;509(7500):366-70
Galectin-8 promotes cytoskeletal rearrangement in trabecular meshwork cells through activation of Rho signaling.
Diskin S, Chen WS, Cao Z, Gyawali S, Gong H, Soza A, González A, Panjwani N
PloS one 2012;7(9):e44400
PloS one 2012;7(9):e44400
Modulation of endothelial cell migration and angiogenesis: a novel function for the "tandem-repeat" lectin galectin-8.
Delgado VM, Nugnes LG, Colombo LL, Troncoso MF, Fernández MM, Malchiodi EL, Frahm I, Croci DO, Compagno D, Rabinovich GA, Wolfenstein-Todel C, Elola MT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):242-54
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Jan;25(1):242-54
Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions.
Cueni LN, Detmar M
Experimental cell research 2009 Jun 10;315(10):1715-23
Experimental cell research 2009 Jun 10;315(10):1715-23
The galectin profile of the endothelium: altered expression and localization in activated and tumor endothelial cells.
Thijssen VL, Hulsmans S, Griffioen AW
The American journal of pathology 2008 Feb;172(2):545-53
The American journal of pathology 2008 Feb;172(2):545-53
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-8 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-8 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-8 by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-8 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Galectin-8 in Human Lung. Galectin-8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.