RQ7184

antibody from NSJ Bioreagents

Targeting: KMO

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7184

Provider

NSJ Bioreagents

Product name

KMO Antibody / Kynurenine 3 monooxygenase

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the KMO antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat kidney, 2) rat liver, 3) mouse kidney and 4) mouse liver tissue lysate with KMO antibody. Predicted molecular weight: 52-56 kDa (multiple isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with KMO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse kidney tissue with KMO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat kidney tissue with KMO antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Daudi cells with KMO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KMO antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse Neuro-2a cells with KMO antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KMO antibody.