PA5-28744

antibody from Invitrogen Antibodies

Targeting: OPN3 ECPN, encephalopsin, ERO, NMO-1, PPP1R116

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28744

Provider

Invitrogen Antibodies

Product name

Opsin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, A431, Jurkat, Raji.

Concentration

1.13 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Opsin 3 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Opsin 3 polyclonal antibody (Product # PA5-28744) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Opsin 3 Polyclonal Antibody (Product # PA5-28744). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Opsin 3 Polyclonal Antibody (Product # PA5-28744) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of Opsin-3 was performed in frozen sectioned adult mouse retina tissue using Opsin 3 Polyclonal Antibody (Product # PA5-28744) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Opsin 3 (Product # PA5-28744) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.