MA5-12262

antibody from Invitrogen Antibodies

Targeting: EXO1 HEX1, hExoI

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12262

Provider

Invitrogen Antibodies

Product name

EXO1 Monoclonal Antibody (266)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-12262 targets Exo1 in WB applications and shows reactivity with Human and mouse samples.

Antibody clone number

266

Concentration

0.2 mg/mL

References

Submitted references

DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response.

Lu H, Saha J, Beckmann PJ, Hendrickson EA, Davis AJ
Nucleic acids research 2019 Oct 10;47(18):9467-9479

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Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.

O'Sullivan RJ, Arnoult N, Lackner DH, Oganesian L, Haggblom C, Corpet A, Almouzni G, Karlseder J
Nature structural & molecular biology 2014 Feb;21(2):167-74

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Exonuclease 1 (Exo1) is required for activating response to S(N)1 DNA methylating agents.

Izumchenko E, Saydi J, Brown KD
DNA repair 2012 Dec 1;11(12):951-64

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Human exonuclease 1 connects nucleotide excision repair (NER) processing with checkpoint activation in response to UV irradiation.

Sertic S, Pizzi S, Cloney R, Lehmann AR, Marini F, Plevani P, Muzi-Falconi M
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 16;108(33):13647-52

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ATR-dependent pathways control hEXO1 stability in response to stalled forks.

El-Shemerly M, Hess D, Pyakurel AK, Moselhy S, Ferrari S
Nucleic acids research 2008 Feb;36(2):511-9

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Degradation of human exonuclease 1b upon DNA synthesis inhibition.

El-Shemerly M, Janscak P, Hess D, Jiricny J, Ferrari S
Cancer research 2005 May 1;65(9):3604-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Exo1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a monoclonal anti-Exo1 antibody (Product # MA5-12262) at a dilution of 1:1000 in 2% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # PI-32209).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Exo1 using Exo1 Monoclonal Antibody (Product # MA5-12262) on MAD109 Cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. DNA-PK cs catalytic activity facilitates the initial recruitment of the DDR machinery to DSBs. ( A ) Recruitment of HR and NHEJ factors to the chromatin after IR is attenuated in the HCT116 DNA-PK cs KD/- cell line. HCT116 DNA-PK cs +/-, -/-, and KD/- cells were mock-treated or irradiated with a dose of 10 Gy and allowed to recover for 10 min. Subsequently, soluble nuclear and the chromatin-enriched fractions were isolated for immunoblotting to assess the recruitment of the proteins listed in the figure to the chromatin after irradiation. ( B ) IR-induced focus formation of MDC1 is attenuated in the HCT116 DNA-PK cs KD/- cell line. The HCT116 DNA-PK cs +/-, -/-, and KD/- cell lines were mock-treated or irradiated with a dose of 1 Gy and MDC1 foci formation was assessed 5 and 10 min later. MDC1 focus formation was examined in at least 50 cells and the number of MDC1 IR-induced foci per nucleus is shown. ****, P value < 0.0001. ( C - F ) Recruitment of GFP-tagged (C) NBS1, (D) EXO1, (E) XLF, and (F) XRCC4 to laser-generated DSBs is attenuated in the HCT116 DNA-PK cs KD/- cell line compared to the +/- cells. Relative fluorescent intensity of GFP-tagged NBS1, EXO1, XLF, and XRCC4 are presented as the mean +- SEM. *, P value < 0.05.