20517-1-AP

antibody from Proteintech Group

Targeting: DESI2 C1orf121, CGI-146, DESI-2, FAM152A, FLJ21998, PNAS-4, PPPDE1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20517-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20517-1-AP, RRID:AB_10696177

Product name

PPPDE1/PNAS4 antibody

Antibody type

Polyclonal

Description

KD/KO validated PPPDE1/PNAS4 antibody (Cat. #20517-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Upregulation of PPPDE1 contributes to anorectal malformations via the mitochondrial apoptosis pathway during hindgut development in rats.

Li SY, Wang CY, Zhao JJ, Long CY, Xiao YX, Tang XB, Yuan ZW, Bai YZ
Experimental cell research 2021 May 15;402(2):112574

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PPPDE1 promotes hepatocellular carcinoma development by negatively regulate p53 and apoptosis.

Xie X, Wang X, Liao W, Fei R, Wu N, Cong X, Chen Q, Wei L, Wang Y, Chen H
Apoptosis : an international journal on programmed cell death 2019 Feb;24(1-2):135-144

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PPPDE1 is a novel deubiquitinase belonging to a cysteine isopeptidase family.

Xie X, Wang X, Jiang D, Wang J, Fei R, Cong X, Wei L, Wang Y, Chen H
Biochemical and biophysical research communications 2017 Jun 24;488(2):291-296

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Analysis of transcription profile to reveal altered signaling pathways following the overexpression of human desumoylating isopeptidase 2 in pancreatic cancer cells.

Fu YY, Kang YH, Shen CC, Wang RX, Yu L, Li XY, Cui DD, Yang JL, Yao YQ, Gou LT
Oncology letters 2016 Dec;12(6):4677-4684

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High Phosphorylation Status of AKT/mTOR Signal in DESI2-Reduced Pancreatic Ductal Adenocarcinoma.

Shen CC, Cui XY, He Y, Kang YH, Yi C, Yang JL, Gou LT
Pathology oncology research : POR 2015 Apr;21(2):267-72

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Implications of PPPDE1 expression in the distribution of plakoglobin and β-catenin in pancreatic ductal adenocarcinoma.

Kang YH, Shen CC, Yao YQ, Yu L, Cui XY, He Y, Yang JL, Gou LT
Oncology letters 2014 Sep;8(3):1229-1233

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Comprehensive analysis of expression profile reveals the ubiquitous distribution of PPPDE peptidase domain 1, a Golgi apparatus component, and its implications in clinical cancer.

He Y, Wang J, Gou L, Shen C, Chen L, Yi C, Wei X, Yang J
Biochimie 2013 Jul;95(7):1466-75

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20517-1-AP(PPPDE1 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PPPDE1 antibody 20517-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 20517-1-AP(PPPDE1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 20517-1-AP(PPPDE1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue