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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP5074D - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP5074d, RRID:AB_10664103
- Product name
- S100B Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant Protein
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using S100B Antibody(Cat. #AP5074d). AP5074d was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of S100B Antibody (Cat. #AP5074d) in mouse liver tissue lysates (35ug/lane).S100B (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- S100B Antibody (Cat. #AP5074d)immunohistochemistry analysis in formalin fixed and paraffin embedded human malignant melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of S100B Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- S100B Antibody (Cat. #AP5074d) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
