676602

antibody from BioLegend

Targeting: S100B S100beta

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

676602

Provider

BioLegend

Proper citation

BioLegend Cat#676602, RRID:AB_2565736

Product name

Purified anti-S100B

Antibody type

Monoclonal

Antigen

Recombinant protein.

Description

S100B is glial-specific. It has been shown that it is only expressed by a subtype of mature astrocytes that ensheath blood vessels, and by NG2-expressing cells. This protein may function in neurite extension, stimulation of Ca2⁺ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing central nervous system (CNS), it acts as a neurotrophic factor and neuronal survival protein. In the adult organism, it is usually elevated due to nervous system damage, which makes it a potential clinical marker. S100B is secreted by astrocytes or can spill from injured cells and enter the extracellular space or bloodstream. Serum levels of S100B increase in patients during the acute phase of brain damage. Over the last decade, S100B has emerged as a candidate peripheral biomarker of blood–brain barrier (BBB) permeability and central nervous system injury.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

11C12E12

Vial size

100 µg

Storage

-20°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot analysis of mouse thymus (negative control) (lane 1), rat brain (lane 2), mouse brain (lane 3), and Aspc-1 (negative control) (lane 4). Anti- S100B antibody (clone 11C12E12) was used, followed by HRP anti-mouse IgG antibody. β-actin antibody was used as a loading control. We are not sure about the specificity of the high molecular weight band.

Conjugate

Unconjugated