V3505

antibody from NSJ Bioreagents

Targeting: S100B S100beta

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3505

Provider

NSJ Bioreagents

Product name

S100B Antibody

Antibody type

Monoclonal

Description

Protein G affinity chromatography

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

S100B/1012

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml

Storage

Store the S100 beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

(Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 beta antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human schwanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human melanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Protein array validation of the S100 beta antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100 beta antibody (clone S100B/1012). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free S100 beta Antibody (clone S100B/1012). Confirmation of Integrity and Purity of the Antibody.