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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB1820 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S100B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human S100B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) S100A1, 2, 6, 7, 10, 11, 13, 16, rmS100A4, 8, 9, or rhS100P.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P04271- Isotype
- IgG
- Antibody clone number
- 472806
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
Qi LY, Xiu MH, Chen DC, Wang F, Kosten TA, Kosten TR, Zhang XY
Neuroscience letters 2009 Sep 22;462(2):113-7
Neuroscience letters 2009 Sep 22;462(2):113-7
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human S100B by Western Blot. Western blot shows lysate of human brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100B Monoclonal Antibody (Catalog # MAB1820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for S100B at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
