Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- PA5-109278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ryanodine Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: 293T, LOVO, SH-SY-5Y, human fetal skeletal muscle tissue, mouse brain tissue, mouse cerebellum tissue, rat skeletal muscle tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RYR2 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RYR2 polyclonal antibody (Product # PA5-109278), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RYR2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RYR2 polyclonal antibody (Product # PA5-109278), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RYR2 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RYR2 polyclonal antibody (Product # PA5-109278), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RYR2 in paraffin-embedded rat skeletal muscle tissue. Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RYR2 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RYR2 in paraffin-embedded human fetal skeletal muscle tissue. Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RYR2 in 293T cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot of RYR2 in PVDF (1 µg, 2 µg and 4 µg of immunization peptides). Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot of RYR2 in PVDF (1 µg, 2 µg and 4 µg of immunization peptides). Samples were incubated with RYR2 polyclonal antibody (Product # PA5-109278) at a dilution of 1:500.