Antibody data
- Antibody Data
- Antigen structure
- References [189]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [10]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA3-916 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ryanodine Receptor Monoclonal Antibody (C3-33)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA3-916 detects ryanodine receptor 2 (RyR2) and shows cross-reactivity with RyR1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.
- Antibody clone number
- C3-33
- Concentration
- 1 mg/mL
Submitted references Anti-inflammatory effects of endothelin receptor blockade in left atrial tissue of spontaneously hypertensive rats.
STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells.
Candesartan Cilexetil Attenuates Arrhythmogenicity Following Pressure Overload in Rats via the Modulation of Cardiac Electrical and Structural Remodeling and Calcium Handling Dysfunction.
Identification of Three Potential Prognostic Genes in Platinum-Resistant Ovarian Cancer via Integrated Bioinformatics Analysis.
Interleukin-6-Mediated-Ca(2+) Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.
Remodeling of t-system and proteins underlying excitation-contraction coupling in aging versus failing human heart.
Phosphorylation-Dependent Interactome of Ryanodine Receptor Type 2 in the Heart.
cAMP Imaging at Ryanodine Receptors Reveals β(2)-Adrenoceptor Driven Arrhythmias.
Cardiac-specific deletion of voltage dependent anion channel 2 leads to dilated cardiomyopathy by altering calcium homeostasis.
Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy.
RyR2 regulates Cx43 hemichannel intracellular Ca2+-dependent activation in cardiomyocytes.
IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency.
Lack of functional wolframin causes drop in plasmalemmal sodium-calcium exchanger type 1 expression at early stage in rat model of Wolfram syndrome.
Structural, Pro-Inflammatory and Calcium Handling Remodeling Underlies Spontaneous Onset of Paroxysmal Atrial Fibrillation in JDP2-Overexpressing Mice.
Increased RyR2 activity is exacerbated by calcium leak-induced mitochondrial ROS.
Chronic dantrolene treatment attenuates cardiac dysfunction and reduces atrial fibrillation inducibility in a rat myocardial infarction heart failure model.
PKA phosphorylation underlies functional recruitment of sarcolemmal SK2 channels in ventricular myocytes from hypertrophic hearts.
Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification.
Phosphorylation of cardiac myosin-binding protein-C contributes to calcium homeostasis.
Molecular characterization of the calcium release channel deficiency syndrome.
Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.
SLMAP3 isoform modulates cardiac gene expression and function.
Impaired Activity of Ryanodine Receptors Contributes to Calcium Mishandling in Cardiomyocytes of Metabolic Syndrome Rats.
Enhancing Autophagy Diminishes Aberrant Ca(2+) Homeostasis and Arrhythmogenesis in Aging Rabbit Hearts.
Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility.
MicroRNA-133 suppresses ZFHX3-dependent atrial remodelling and arrhythmia.
Role of Ryanodine Type 2 Receptors in Elementary Ca(2+) Signaling in Arteries and Vascular Adaptive Responses.
Ion channel gating in cardiac ryanodine receptors from the arrhythmic RyR2-P2328S mouse.
3D dSTORM imaging reveals novel detail of ryanodine receptor localization in rat cardiac myocytes.
Control of Neuronal Ryanodine Receptor-Mediated Calcium Signaling by Calsenilin.
Adverse transverse-tubule remodeling in a rat model of heart failure is attenuated with low-dose triiodothyronine treatment.
Cardiomyocyte substructure reverts to an immature phenotype during heart failure.
Iron-deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity.
Empagliflozin Attenuates Myocardial Sodium and Calcium Dysregulation and Reverses Cardiac Remodeling in Streptozotocin-Induced Diabetic Rats.
Pharmacological Modulation of Mitochondrial Ca(2+) Content Regulates Sarcoplasmic Reticulum Ca(2+) Release via Oxidation of the Ryanodine Receptor by Mitochondria-Derived Reactive Oxygen Species.
Profibrotic, Electrical, and Calcium-Handling Remodeling of the Atria in Heart Failure Patients With and Without Atrial Fibrillation.
Axial Tubule Junctions Activate Atrial Ca(2+) Release Across Species.
N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer's Disease Rat Model.
Long-term contractile activity and thyroid hormone supplementation produce engineered rat myocardium with adult-like structure and function.
The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy.
Ryanodine receptor dispersion disrupts Ca(2+) release in failing cardiac myocytes.
Calcium Release Mediated by Redox-Sensitive RyR2 Channels Has a Central Role in Hippocampal Structural Plasticity and Spatial Memory.
True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors.
Highly variable contractile performance correlates with myocyte content in trabeculae from failing human hearts.
Osteolytic Breast Cancer Causes Skeletal Muscle Weakness in an Immunocompetent Syngeneic Mouse Model.
MG53 is dispensable for T-tubule maturation but critical for maintaining T-tubule integrity following cardiac stress.
Muscular dystrophy in PTFR/cavin-1 null mice.
Notch Inhibition Enhances Cardiac Reprogramming by Increasing MEF2C Transcriptional Activity.
SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR.
Subcellular localization of Na/K-ATPase isoforms in ventricular myocytes.
Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm.
Sarcolemmal distribution of I(Ca) and I(NCX) and Ca(2+) autoregulation in mouse ventricular myocytes.
SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Heart failure modulates electropharmacological characteristics of sinoatrial nodes.
A protocol for registration and correction of multicolour STED superresolution images.
Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women.
Sheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading.
RyR2R420Q catecholaminergic polymorphic ventricular tachycardia mutation induces bradycardia by disturbing the coupled clock pacemaker mechanism.
Progesterone Protects Against Bisphenol A-Induced Arrhythmias in Female Rat Cardiac Myocytes via Rapid Signaling.
Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.
CaMKII-dependent phosphorylation regulates basal cardiac pacemaker function via modulation of local Ca2+ releases.
Strategic Positioning and Biased Activity of the Mitochondrial Calcium Uniporter in Cardiac Muscle.
Expression of connexin 43, ion channels and Ca(2+)-handling proteins in rat pulmonary vein cardiomyocytes.
The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a.
Heterozygous deletion of sarcolipin maintains normal cardiac function.
Early transverse tubule development begins in utero in the sheep heart.
Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes.
Inhibition of Rac1 reduces store overload-induced calcium release and protects against ventricular arrhythmia.
Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells.
Cellular Hypertrophy and Increased Susceptibility to Spontaneous Calcium-Release of Rat Left Atrial Myocytes Due to Elevated Afterload.
Altered distribution of ICa impairs Ca release at the t-tubules of ventricular myocytes from failing hearts.
Examination of the Effects of Heterogeneous Organization of RyR Clusters, Myofibrils and Mitochondria on Ca2+ Release Patterns in Cardiomyocytes.
Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging.
Cardiac Resynchronization Therapy Reduces Subcellular Heterogeneity of Ryanodine Receptors, T-Tubules, and Ca2+ Sparks Produced by Dyssynchronous Heart Failure.
Excess TGF-β mediates muscle weakness associated with bone metastases in mice.
Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes.
RyR2 modulates a Ca2+-activated K+ current in mouse cardiac myocytes.
Hierarchical clustering of ryanodine receptors enables emergence of a calcium clock in sinoatrial node cells.
TRPV2 is critical for the maintenance of cardiac structure and function in mice.
Age-associated abnormalities of intrinsic automaticity of sinoatrial nodal cells are linked to deficient cAMP-PKA-Ca(2+) signaling.
Suppression of InsP3 receptor-mediated Ca2+ signaling alleviates mutant presenilin-linked familial Alzheimer's disease pathogenesis.
A modified local control model for Ca2+ transients in cardiomyocytes: junctional flux is accompanied by release from adjacent non-junctional RyRs.
Oxidation- and CaMKII-mediated sarcoplasmic reticulum Ca(2+) leak triggers atrial fibrillation in aging.
Single delivery of an adeno-associated viral construct to transfer the CASQ2 gene to knock-in mice affected by catecholaminergic polymorphic ventricular tachycardia is able to cure the disease from birth to advanced age.
Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure.
Loss of microRNA-106b-25 cluster promotes atrial fibrillation by enhancing ryanodine receptor type-2 expression and calcium release.
Reduced aerobic capacity causes leaky ryanodine receptors that trigger arrhythmia in a rat strain artificially selected and bred for low aerobic running capacity.
Reduced IRE1α mediates apoptotic cell death by disrupting calcium homeostasis via the InsP3 receptor.
Cardio-protection by Ginkgo biloba extract 50 in rats with acute myocardial infarction is related to Na⁺-Ca²⁺ exchanger.
Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.
Inhibition of the late sodium current slows t-tubule disruption during the progression of hypertensive heart disease in the rat.
Cardiomyocyte ryanodine receptor degradation by chaperone-mediated autophagy.
Phospholipase Cε hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy.
Bradycardia alters Ca(2+) dynamics enhancing dispersion of repolarization and arrhythmia risk.
Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons.
Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere.
Large-conductance calcium-activated potassium current modulates excitability in isolated canine intracardiac neurons.
Ionic bases for electrical remodeling of the canine cardiac ventricle.
Advanced glycation end product cross-link breaker attenuates diabetes-induced cardiac dysfunction by improving sarcoplasmic reticulum calcium handling.
JTV519 (K201) reduces sarcoplasmic reticulum Ca²⁺ leak and improves diastolic function in vitro in murine and human non-failing myocardium.
Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart.
Zinc-induced cardiomyocyte relaxation in a rat model of hyperglycemia is independent of myosin isoform.
Coupling of ryanodine receptor 2 and voltage-dependent anion channel 2 is essential for Ca²+ transfer from the sarcoplasmic reticulum to the mitochondria in the heart.
Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Extreme sarcoplasmic reticulum volume loss and compensatory T-tubule remodeling after Serca2 knockout.
Amyloid peptide regulates calcium homoeostasis and arrhythmogenesis in pulmonary vein cardiomyocytes.
Probenecid: novel use as a non-injurious positive inotrope acting via cardiac TRPV2 stimulation.
Overexpression of histidine-rich calcium binding protein in equine ventricular myocardium.
Combined insulin treatment and intense exercise training improved basal cardiac function and Ca(2+)-cycling proteins expression in type 1 diabetic rats.
ACE inhibition prevents diastolic Ca2+ overload and loss of myofilament Ca2+ sensitivity after myocardial infarction.
Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.
Ca²+-regulatory proteins in cardiomyocytes from the right ventricle in children with congenital heart disease.
4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.
Cardiac calcium pump inactivation and nitrosylation in senescent rat myocardium are not attenuated by long-term treadmill training.
FKBP12.6 mice display temporal gender differences in cardiac Ca(2+)-signalling phenotype upon chronic pressure overload.
Ca(2+)-related signaling and protein phosphorylation abnormalities play central roles in a new experimental model of electrical storm.
Changes in the organization of excitation-contraction coupling structures in failing human heart.
Exercise training does not improve cardiac function in compensated or decompensated left ventricular hypertrophy induced by aortic stenosis.
Loss of the AE3 anion exchanger in a hypertrophic cardiomyopathy model causes rapid decompensation and heart failure.
Effects of exercise training combined with insulin treatment on cardiac NOS1 signaling pathways in type 1 diabetic rats.
Reduced Ca2+ spark activity after subarachnoid hemorrhage disables BK channel control of cerebral artery tone.
Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity and spatial memory formation.
Couplons in rat atria form distinct subgroups defined by their molecular partners.
Multi-image colocalization and its statistical significance.
Sequential alterations in Akt, GSK3β, and calcineurin signalling in the mouse left ventricle after thoracic aortic constriction.
A new twist in cardiac muscle: dislocated and helicoid arrangements of myofibrillar z-disks in mammalian ventricular myocytes.
Chronic heart rate reduction with ivabradine improves systolic function of the reperfused heart through a dual mechanism involving a direct mechanical effect and a long-term increase in FKBP12/12.6 expression.
A novel ryanodine receptor expressed in pancreatic islets by alternative splicing from type 2 ryanodine receptor gene.
Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway.
Phospholamban ablation rescues sarcoplasmic reticulum Ca(2+) handling but exacerbates cardiac dysfunction in CaMKIIdelta(C) transgenic mice.
Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure.
Regulation of myocyte contraction via neuronal nitric oxide synthase: role of ryanodine receptor S-nitrosylation.
Defective calmodulin binding to the cardiac ryanodine receptor plays a key role in CPVT-associated channel dysfunction.
Distribution of ryanodine receptors in rat ventricular myocytes.
Three-dimensional high-resolution imaging of cardiac proteins to construct models of intracellular Ca2+ signalling in rat ventricular myocytes.
Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy.
Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia.
The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca2+.
Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes.
Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.
Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation.
Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle.
Differential alterations in expressions of ryanodine receptor subtypes in cerebellar cortical neurons of an ataxic mutant, rolling mouse Nagoya.
IP3 receptor-dependent Ca2+ release modulates excitation-contraction coupling in rabbit ventricular myocytes.
Wide long lasting perinuclear Ca2+ release events generated by an interaction between ryanodine and IP3 receptors in canine Purkinje cells.
Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model.
Three-dimensional distribution of cardiac Na+-Ca2+ exchanger and ryanodine receptor during development.
Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms.
CaMKII-mediated increased lusitropic responses to beta-adrenoreceptor stimulation in ANP-receptor deficient mice.
Thermogenic activity of Ca2+-ATPase from skeletal muscle heavy sarcoplasmic reticulum: the role of ryanodine Ca2+ channel.
Retina expresses a novel variant of the ryanodine receptor.
Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells.
Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus.
Functional groups of ryanodine receptors in rat ventricular cells.
Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins.
New cardioprotective agent K201 is natriuretic and glomerular filtration rate enhancing.
Increased sarcoplasmic reticulum calcium leak but unaltered contractility by acute CaMKII overexpression in isolated rabbit cardiac myocytes.
Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein.
Caveolin-3 is adjacent to a group of extradyadic ryanodine receptors.
Ca2+ sparks and waves in canine purkinje cells: a triple layered system of Ca2+ activation.
Distinct subcellular location of the Ca2+-binding protein S100A1 differentially modulates Ca2+-cycling in ventricular rat cardiomyocytes.
Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes.
RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets.
Calcium/calmodulin-dependent protein kinase IIdelta associates with the ryanodine receptor complex and regulates channel function in rabbit heart.
Dilated cardiomyopathy caused by tissue-specific ablation of SC35 in the heart.
Identification of biochemical adaptations in hyper- or hypocontractile hearts from phospholamban mutant mice by expression proteomics.
ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors.
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation.
Ca2+ regulatory systems in rat myocardium are altered by 24 weeks treadmill training.
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytes.
The ryanodine receptor modulates the spontaneous beating rate of cardiomyocytes during development.
Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling.
Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons.
cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells.
Ryanodine receptor dysfunction in hearts of streptozotocin-induced diabetic rats.
Regulation of aquaporin-2 trafficking by vasopressin in the renal collecting duct. Roles of ryanodine-sensitive Ca2+ stores and calmodulin.
Intracellular Ca(2+) handling in vascular smooth muscle cells is affected by proliferation.
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum.
Reversible block of the calcium release channel/ryanodine receptor by protamine, a heparin antidote.
Expression and functional characterization of the cardiac muscle ryanodine receptor Ca(2+) release channel in Chinese hamster ovary cells.
Ontogeny of local sarcoplasmic reticulum Ca2+ signals in cerebral arteries: Ca2+ sparks as elementary physiological events.
A-kinase anchoring protein 100 (AKAP100) is localized in multiple subcellular compartments in the adult rat heart.
Detection and functional characterization of ryanodine receptors from sea urchin eggs.
Characterization of recombinant rabbit cardiac and skeletal muscle Ca2+ release channels (ryanodine receptors) with a novel [3H]ryanodine binding assay.
Distribution of inositol-1,4,5-trisphosphate and ryanodine receptors in rat neostriatum.
Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts.
Expression of a cardiac Ca(2+)-release channel isoform in mammalian brain.
Bukowska A, Nikonova Y, Wolke C, Lendeckel U, Kockskämper J, Goette A
International journal of cardiology. Heart & vasculature 2022 Oct;42:101088
International journal of cardiology. Heart & vasculature 2022 Oct;42:101088
STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells.
Krishnan V, Ali S, Gonzales AL, Thakore P, Griffin CS, Yamasaki E, Alvarado MG, Johnson MT, Trebak M, Earley S
eLife 2022 Feb 11;11
eLife 2022 Feb 11;11
Candesartan Cilexetil Attenuates Arrhythmogenicity Following Pressure Overload in Rats via the Modulation of Cardiac Electrical and Structural Remodeling and Calcium Handling Dysfunction.
Chang GJ, Yeh YH, Chen WJ, Ko YS, Lai YJ, Lee YS
Journal of the American Heart Association 2022 Aug 2;11(15):e024285
Journal of the American Heart Association 2022 Aug 2;11(15):e024285
Identification of Three Potential Prognostic Genes in Platinum-Resistant Ovarian Cancer via Integrated Bioinformatics Analysis.
Zhang X, Wei X, Bai G, Huang X, Hu S, Mao H, Liu P
Cancer management and research 2021;13:8629-8646
Cancer management and research 2021;13:8629-8646
Interleukin-6-Mediated-Ca(2+) Handling Abnormalities Contributes to Atrial Fibrillation in Sterile Pericarditis Rats.
Liao J, Zhang S, Yang S, Lu Y, Lu K, Wu Y, Wu Q, Zhao N, Dong Q, Chen L, Du Y
Frontiers in immunology 2021;12:758157
Frontiers in immunology 2021;12:758157
Remodeling of t-system and proteins underlying excitation-contraction coupling in aging versus failing human heart.
Lyu Y, Verma VK, Lee Y, Taleb I, Badolia R, Shankar TS, Kyriakopoulos CP, Selzman CH, Caine W, Alharethi R, Navankasattusas S, Seidel T, Drakos SG, Sachse FB
NPJ aging and mechanisms of disease 2021 May 28;7(1):16
NPJ aging and mechanisms of disease 2021 May 28;7(1):16
Phosphorylation-Dependent Interactome of Ryanodine Receptor Type 2 in the Heart.
Chiang DY, Lahiri S, Wang G, Karch J, Wang MC, Jung SY, Heck AJR, Scholten A, Wehrens XHT
Proteomes 2021 Jun 7;9(2)
Proteomes 2021 Jun 7;9(2)
cAMP Imaging at Ryanodine Receptors Reveals β(2)-Adrenoceptor Driven Arrhythmias.
Berisha F, Götz KR, Wegener JW, Brandenburg S, Subramanian H, Molina CE, Rüffer A, Petersen J, Bernhardt A, Girdauskas E, Jungen C, Pape U, Kraft AE, Warnke S, Lindner D, Westermann D, Blankenberg S, Meyer C, Hasenfuß G, Lehnart SE, Nikolaev VO
Circulation research 2021 Jun 25;129(1):81-94
Circulation research 2021 Jun 25;129(1):81-94
Cardiac-specific deletion of voltage dependent anion channel 2 leads to dilated cardiomyopathy by altering calcium homeostasis.
Shankar TS, Ramadurai DKA, Steinhorst K, Sommakia S, Badolia R, Thodou Krokidi A, Calder D, Navankasattusas S, Sander P, Kwon OS, Aravamudhan A, Ling J, Dendorfer A, Xie C, Kwon O, Cheng EHY, Whitehead KJ, Gudermann T, Richardson RS, Sachse FB, Schredelseker J, Spitzer KW, Chaudhuri D, Drakos SG
Nature communications 2021 Jul 28;12(1):4583
Nature communications 2021 Jul 28;12(1):4583
Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy.
Clowsley AH, Kaufhold WT, Lutz T, Meletiou A, Di Michele L, Soeller C
Nature communications 2021 Jan 21;12(1):501
Nature communications 2021 Jan 21;12(1):501
RyR2 regulates Cx43 hemichannel intracellular Ca2+-dependent activation in cardiomyocytes.
Lissoni A, Hulpiau P, Martins-Marques T, Wang N, Bultynck G, Schulz R, Witschas K, Girao H, De Smet M, Leybaert L
Cardiovascular research 2021 Jan 1;117(1):123-136
Cardiovascular research 2021 Jan 1;117(1):123-136
IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency.
Luo X, Li W, Künzel K, Henze S, Cyganek L, Strano A, Poetsch MS, Schubert M, Guan K
Frontiers in cell and developmental biology 2020;8:772
Frontiers in cell and developmental biology 2020;8:772
Lack of functional wolframin causes drop in plasmalemmal sodium-calcium exchanger type 1 expression at early stage in rat model of Wolfram syndrome.
Kureková S, Plaas M, Cagalinec M
General physiology and biophysics 2020 Sep;39(5):499-503
General physiology and biophysics 2020 Sep;39(5):499-503
Structural, Pro-Inflammatory and Calcium Handling Remodeling Underlies Spontaneous Onset of Paroxysmal Atrial Fibrillation in JDP2-Overexpressing Mice.
Parahuleva MS, Kockskämper J, Heger J, Grimm W, Scherer A, Bühler S, Kreutz J, Schulz R, Euler G
International journal of molecular sciences 2020 Nov 30;21(23)
International journal of molecular sciences 2020 Nov 30;21(23)
Increased RyR2 activity is exacerbated by calcium leak-induced mitochondrial ROS.
Hamilton S, Terentyeva R, Martin B, Perger F, Li J, Stepanov A, Bonilla IM, Knollmann BC, Radwański PB, Györke S, Belevych AE, Terentyev D
Basic research in cardiology 2020 May 22;115(4):38
Basic research in cardiology 2020 May 22;115(4):38
Chronic dantrolene treatment attenuates cardiac dysfunction and reduces atrial fibrillation inducibility in a rat myocardial infarction heart failure model.
Nofi C, Zhang K, Tang YD, Li Y, Migirov A, Ojamaa K, Gerdes AM, Zhang Y
Heart rhythm O2 2020 Jun;1(2):126-135
Heart rhythm O2 2020 Jun;1(2):126-135
PKA phosphorylation underlies functional recruitment of sarcolemmal SK2 channels in ventricular myocytes from hypertrophic hearts.
Hamilton S, Polina I, Terentyeva R, Bronk P, Kim TY, Roder K, Clements RT, Koren G, Choi BR, Terentyev D
The Journal of physiology 2020 Jul;598(14):2847-2873
The Journal of physiology 2020 Jul;598(14):2847-2873
Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification.
Asghari P, Scriven DR, Ng M, Panwar P, Chou KC, van Petegem F, Moore ED
eLife 2020 Jan 9;9
eLife 2020 Jan 9;9
Phosphorylation of cardiac myosin-binding protein-C contributes to calcium homeostasis.
Kumar M, Haghighi K, Kranias EG, Sadayappan S
The Journal of biological chemistry 2020 Aug 7;295(32):11275-11291
The Journal of biological chemistry 2020 Aug 7;295(32):11275-11291
Molecular characterization of the calcium release channel deficiency syndrome.
Tester DJ, Kim CSJ, Hamrick SK, Ye D, O'Hare BJ, Bombei HM, Fitzgerald KK, Haglund-Turnquist CM, Atkins DL, Nunez LAO, Law I, Temple J, Ackerman MJ
JCI insight 2020 Aug 6;5(15)
JCI insight 2020 Aug 6;5(15)
Altered calcium handling produces reentry-promoting action potential alternans in atrial fibrillation-remodeled hearts.
Liu T, Xiong F, Qi XY, Xiao J, Villeneuve L, Abu-Taha I, Dobrev D, Huang C, Nattel S
JCI insight 2020 Apr 7;5(8)
JCI insight 2020 Apr 7;5(8)
SLMAP3 isoform modulates cardiac gene expression and function.
Mlynarova J, Trentin-Sonoda M, Gaisler da Silva F, Major JL, Salih M, Carneiro-Ramos MS, Tuana BS
PloS one 2019;14(4):e0214669
PloS one 2019;14(4):e0214669
Impaired Activity of Ryanodine Receptors Contributes to Calcium Mishandling in Cardiomyocytes of Metabolic Syndrome Rats.
Fernández-Miranda G, Romero-Garcia T, Barrera-Lechuga TP, Mercado-Morales M, Rueda A
Frontiers in physiology 2019;10:520
Frontiers in physiology 2019;10:520
Enhancing Autophagy Diminishes Aberrant Ca(2+) Homeostasis and Arrhythmogenesis in Aging Rabbit Hearts.
Murphy KR, Baggett B, Cooper LL, Lu Y, O-Uchi J, Sedivy JM, Terentyev D, Koren G
Frontiers in physiology 2019;10:1277
Frontiers in physiology 2019;10:1277
Nanoscale coupling of junctophilin-2 and ryanodine receptors regulates vascular smooth muscle cell contractility.
Pritchard HAT, Griffin CS, Yamasaki E, Thakore P, Lane C, Greenstein AS, Earley S
Proceedings of the National Academy of Sciences of the United States of America 2019 Oct 22;116(43):21874-21881
Proceedings of the National Academy of Sciences of the United States of America 2019 Oct 22;116(43):21874-21881
MicroRNA-133 suppresses ZFHX3-dependent atrial remodelling and arrhythmia.
Cheng WL, Kao YH, Chao TF, Lin YK, Chen SA, Chen YJ
Acta physiologica (Oxford, England) 2019 Nov;227(3):e13322
Acta physiologica (Oxford, England) 2019 Nov;227(3):e13322
Role of Ryanodine Type 2 Receptors in Elementary Ca(2+) Signaling in Arteries and Vascular Adaptive Responses.
Kaßmann M, Szijártó IA, García-Prieto CF, Fan G, Schleifenbaum J, Anistan YM, Tabeling C, Shi Y, le Noble F, Witzenrath M, Huang Y, Markó L, Nelson MT, Gollasch M
Journal of the American Heart Association 2019 May 7;8(9):e010090
Journal of the American Heart Association 2019 May 7;8(9):e010090
Ion channel gating in cardiac ryanodine receptors from the arrhythmic RyR2-P2328S mouse.
Salvage SC, Gallant EM, Beard NA, Ahmad S, Valli H, Fraser JA, Huang CL, Dulhunty AF
Journal of cell science 2019 May 21;132(10)
Journal of cell science 2019 May 21;132(10)
3D dSTORM imaging reveals novel detail of ryanodine receptor localization in rat cardiac myocytes.
Shen X, van den Brink J, Hou Y, Colli D, Le C, Kolstad TR, MacQuaide N, Carlson CR, Kekenes-Huskey PM, Edwards AG, Soeller C, Louch WE
The Journal of physiology 2019 Jan;597(2):399-418
The Journal of physiology 2019 Jan;597(2):399-418
Control of Neuronal Ryanodine Receptor-Mediated Calcium Signaling by Calsenilin.
Grillo MA, Grillo SL, Gerdes BC, Kraus JG, Koulen P
Molecular neurobiology 2019 Jan;56(1):525-534
Molecular neurobiology 2019 Jan;56(1):525-534
Adverse transverse-tubule remodeling in a rat model of heart failure is attenuated with low-dose triiodothyronine treatment.
An S, Gilani N, Huang Y, Muncan A, Zhang Y, Tang YD, Gerdes AM, Ojamaa K
Molecular medicine (Cambridge, Mass.) 2019 Dec 6;25(1):53
Molecular medicine (Cambridge, Mass.) 2019 Dec 6;25(1):53
Cardiomyocyte substructure reverts to an immature phenotype during heart failure.
Lipsett DB, Frisk M, Aronsen JM, Nordén ES, Buonarati OR, Cataliotti A, Hell JW, Sjaastad I, Christensen G, Louch WE
The Journal of physiology 2019 Apr;597(7):1833-1853
The Journal of physiology 2019 Apr;597(7):1833-1853
Iron-deficiency anemia reduces cardiac contraction by downregulating RyR2 channels and suppressing SERCA pump activity.
Chung YJ, Luo A, Park KC, Loonat AA, Lakhal-Littleton S, Robbins PA, Swietach P
JCI insight 2019 Apr 4;4(7)
JCI insight 2019 Apr 4;4(7)
Empagliflozin Attenuates Myocardial Sodium and Calcium Dysregulation and Reverses Cardiac Remodeling in Streptozotocin-Induced Diabetic Rats.
Lee TI, Chen YC, Lin YK, Chung CC, Lu YY, Kao YH, Chen YJ
International journal of molecular sciences 2019 Apr 4;20(7)
International journal of molecular sciences 2019 Apr 4;20(7)
Pharmacological Modulation of Mitochondrial Ca(2+) Content Regulates Sarcoplasmic Reticulum Ca(2+) Release via Oxidation of the Ryanodine Receptor by Mitochondria-Derived Reactive Oxygen Species.
Hamilton S, Terentyeva R, Kim TY, Bronk P, Clements RT, O-Uchi J, Csordás G, Choi BR, Terentyev D
Frontiers in physiology 2018;9:1831
Frontiers in physiology 2018;9:1831
Profibrotic, Electrical, and Calcium-Handling Remodeling of the Atria in Heart Failure Patients With and Without Atrial Fibrillation.
Molina CE, Abu-Taha IH, Wang Q, Roselló-Díez E, Kamler M, Nattel S, Ravens U, Wehrens XHT, Hove-Madsen L, Heijman J, Dobrev D
Frontiers in physiology 2018;9:1383
Frontiers in physiology 2018;9:1383
Axial Tubule Junctions Activate Atrial Ca(2+) Release Across Species.
Brandenburg S, Pawlowitz J, Fakuade FE, Kownatzki-Danger D, Kohl T, Mitronova GY, Scardigli M, Neef J, Schmidt C, Wiedmann F, Pavone FS, Sacconi L, Kutschka I, Sossalla S, Moser T, Voigt N, Lehnart SE
Frontiers in physiology 2018;9:1227
Frontiers in physiology 2018;9:1227
N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer's Disease Rat Model.
More J, Galusso N, Veloso P, Montecinos L, Finkelstein JP, Sanchez G, Bull R, Valdés JL, Hidalgo C, Paula-Lima A
Frontiers in aging neuroscience 2018;10:399
Frontiers in aging neuroscience 2018;10:399
Long-term contractile activity and thyroid hormone supplementation produce engineered rat myocardium with adult-like structure and function.
Jackman C, Li H, Bursac N
Acta biomaterialia 2018 Sep 15;78:98-110
Acta biomaterialia 2018 Sep 15;78:98-110
The DWORF micropeptide enhances contractility and prevents heart failure in a mouse model of dilated cardiomyopathy.
Makarewich CA, Munir AZ, Schiattarella GG, Bezprozvannaya S, Raguimova ON, Cho EE, Vidal AH, Robia SL, Bassel-Duby R, Olson EN
eLife 2018 Oct 9;7
eLife 2018 Oct 9;7
Ryanodine receptor dispersion disrupts Ca(2+) release in failing cardiac myocytes.
Kolstad TR, van den Brink J, MacQuaide N, Lunde PK, Frisk M, Aronsen JM, Norden ES, Cataliotti A, Sjaastad I, Sejersted OM, Edwards AG, Lines GT, Louch WE
eLife 2018 Oct 30;7
eLife 2018 Oct 30;7
Calcium Release Mediated by Redox-Sensitive RyR2 Channels Has a Central Role in Hippocampal Structural Plasticity and Spatial Memory.
More JY, Bruna BA, Lobos PE, Galaz JL, Figueroa PL, Namias S, Sánchez GL, Barrientos GC, Valdés JL, Paula-Lima AC, Hidalgo C, Adasme T
Antioxidants & redox signaling 2018 Oct 20;29(12):1125-1146
Antioxidants & redox signaling 2018 Oct 20;29(12):1125-1146
True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors.
Jayasinghe I, Clowsley AH, Lin R, Lutz T, Harrison C, Green E, Baddeley D, Di Michele L, Soeller C
Cell reports 2018 Jan 9;22(2):557-567
Cell reports 2018 Jan 9;22(2):557-567
Highly variable contractile performance correlates with myocyte content in trabeculae from failing human hearts.
Munro ML, Shen X, Ward M, Ruygrok PN, Crossman DJ, Soeller C
Scientific reports 2018 Feb 13;8(1):2957
Scientific reports 2018 Feb 13;8(1):2957
Osteolytic Breast Cancer Causes Skeletal Muscle Weakness in an Immunocompetent Syngeneic Mouse Model.
Regan JN, Mikesell C, Reiken S, Xu H, Marks AR, Mohammad KS, Guise TA, Waning DL
Frontiers in endocrinology 2017;8:358
Frontiers in endocrinology 2017;8:358
MG53 is dispensable for T-tubule maturation but critical for maintaining T-tubule integrity following cardiac stress.
Zhang C, Chen B, Wang Y, Guo A, Tang Y, Khataei T, Shi Y, Kutschke WJ, Zimmerman K, Weiss RM, Liu J, Benson CJ, Hong J, Ma J, Song LS
Journal of molecular and cellular cardiology 2017 Nov;112:123-130
Journal of molecular and cellular cardiology 2017 Nov;112:123-130
Muscular dystrophy in PTFR/cavin-1 null mice.
Ding SY, Liu L, Pilch PF
JCI insight 2017 Mar 9;2(5):e91023
JCI insight 2017 Mar 9;2(5):e91023
Notch Inhibition Enhances Cardiac Reprogramming by Increasing MEF2C Transcriptional Activity.
Abad M, Hashimoto H, Zhou H, Morales MG, Chen B, Bassel-Duby R, Olson EN
Stem cell reports 2017 Mar 14;8(3):548-560
Stem cell reports 2017 Mar 14;8(3):548-560
SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR.
Kim TY, Terentyeva R, Roder KH, Li W, Liu M, Greener I, Hamilton S, Polina I, Murphy KR, Clements RT, Dudley SC Jr, Koren G, Choi BR, Terentyev D
Cardiovascular research 2017 Mar 1;113(3):343-353
Cardiovascular research 2017 Mar 1;113(3):343-353
Subcellular localization of Na/K-ATPase isoforms in ventricular myocytes.
Yuen GK, Galice S, Bers DM
Journal of molecular and cellular cardiology 2017 Jul;108:158-169
Journal of molecular and cellular cardiology 2017 Jul;108:158-169
Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm.
Cerrone M, Montnach J, Lin X, Zhao YT, Zhang M, Agullo-Pascual E, Leo-Macias A, Alvarado FJ, Dolgalev I, Karathanos TV, Malkani K, Van Opbergen CJM, van Bavel JJA, Yang HQ, Vasquez C, Tester D, Fowler S, Liang F, Rothenberg E, Heguy A, Morley GE, Coetzee WA, Trayanova NA, Ackerman MJ, van Veen TAB, Valdivia HH, Delmar M
Nature communications 2017 Jul 24;8(1):106
Nature communications 2017 Jul 24;8(1):106
Sarcolemmal distribution of I(Ca) and I(NCX) and Ca(2+) autoregulation in mouse ventricular myocytes.
Gadeberg HC, Kong CHT, Bryant SM, James AF, Orchard CH
American journal of physiology. Heart and circulatory physiology 2017 Jul 1;313(1):H190-H199
American journal of physiology. Heart and circulatory physiology 2017 Jul 1;313(1):H190-H199
SPEG (Striated Muscle Preferentially Expressed Protein Kinase) Is Essential for Cardiac Function by Regulating Junctional Membrane Complex Activity.
Quick AP, Wang Q, Philippen LE, Barreto-Torres G, Chiang DY, Beavers D, Wang G, Khalid M, Reynolds JO, Campbell HM, Showell J, McCauley MD, Scholten A, Wehrens XH
Circulation research 2017 Jan 6;120(1):110-119
Circulation research 2017 Jan 6;120(1):110-119
Heart failure modulates electropharmacological characteristics of sinoatrial nodes.
Chang SL, Chuang HL, Chen YC, Kao YH, Lin YK, Yeh YH, Chen SA, Chen YJ
Experimental and therapeutic medicine 2017 Feb;13(2):771-779
Experimental and therapeutic medicine 2017 Feb;13(2):771-779
A protocol for registration and correction of multicolour STED superresolution images.
Hebisch E, Wagner E, Westphal V, Sieber JJ, Lehnart SE
Journal of microscopy 2017 Aug;267(2):160-175
Journal of microscopy 2017 Aug;267(2):160-175
Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women.
Papp R, Bett GCL, Lis A, Rasmusson RL, Baczkó I, Varró A, Salama G
Biology of sex differences 2017 Aug 14;8(1):26
Biology of sex differences 2017 Aug 14;8(1):26
Sheet-Like Remodeling of the Transverse Tubular System in Human Heart Failure Impairs Excitation-Contraction Coupling and Functional Recovery by Mechanical Unloading.
Seidel T, Navankasattusas S, Ahmad A, Diakos NA, Xu WD, Tristani-Firouzi M, Bonios MJ, Taleb I, Li DY, Selzman CH, Drakos SG, Sachse FB
Circulation 2017 Apr 25;135(17):1632-1645
Circulation 2017 Apr 25;135(17):1632-1645
RyR2R420Q catecholaminergic polymorphic ventricular tachycardia mutation induces bradycardia by disturbing the coupled clock pacemaker mechanism.
Wang YY, Mesirca P, Marqués-Sulé E, Zahradnikova A Jr, Villejoubert O, D'Ocon P, Ruiz C, Domingo D, Zorio E, Mangoni ME, Benitah JP, Gómez AM
JCI insight 2017 Apr 20;2(8)
JCI insight 2017 Apr 20;2(8)
Progesterone Protects Against Bisphenol A-Induced Arrhythmias in Female Rat Cardiac Myocytes via Rapid Signaling.
Ma J, Hong K, Wang HS
Endocrinology 2017 Apr 1;158(4):778-790
Endocrinology 2017 Apr 1;158(4):778-790
Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure.
Sadredini M, Danielsen TK, Aronsen JM, Manotheepan R, Hougen K, Sjaastad I, Stokke MK
PloS one 2016;11(4):e0153887
PloS one 2016;11(4):e0153887
CaMKII-dependent phosphorylation regulates basal cardiac pacemaker function via modulation of local Ca2+ releases.
Li Y, Sirenko S, Riordon DR, Yang D, Spurgeon H, Lakatta EG, Vinogradova TM
American journal of physiology. Heart and circulatory physiology 2016 Sep 1;311(3):H532-44
American journal of physiology. Heart and circulatory physiology 2016 Sep 1;311(3):H532-44
Strategic Positioning and Biased Activity of the Mitochondrial Calcium Uniporter in Cardiac Muscle.
De La Fuente S, Fernandez-Sanz C, Vail C, Agra EJ, Holmstrom K, Sun J, Mishra J, Williams D, Finkel T, Murphy E, Joseph SK, Sheu SS, Csordás G
The Journal of biological chemistry 2016 Oct 28;291(44):23343-23362
The Journal of biological chemistry 2016 Oct 28;291(44):23343-23362
Expression of connexin 43, ion channels and Ca(2+)-handling proteins in rat pulmonary vein cardiomyocytes.
Xiao Y, Cai X, Atkinson A, Logantha SJ, Boyett M, Dobrzynski H
Experimental and therapeutic medicine 2016 Nov;12(5):3233-3241
Experimental and therapeutic medicine 2016 Nov;12(5):3233-3241
The angiotensin receptor-associated protein Atrap is a stimulator of the cardiac Ca2+-ATPase SERCA2a.
Mederle K, Gess B, Pluteanu F, Plackic J, Tiefenbach KJ, Grill A, Kockskämper J, Castrop H
Cardiovascular research 2016 Jun 1;110(3):359-70
Cardiovascular research 2016 Jun 1;110(3):359-70
Heterozygous deletion of sarcolipin maintains normal cardiac function.
Shimura D, Kusakari Y, Sasano T, Nakashima Y, Nakai G, Jiao Q, Jin M, Yokota T, Ishikawa Y, Nakano A, Goda N, Minamisawa S
American journal of physiology. Heart and circulatory physiology 2016 Jan 1;310(1):H92-103
American journal of physiology. Heart and circulatory physiology 2016 Jan 1;310(1):H92-103
Early transverse tubule development begins in utero in the sheep heart.
Munro ML, Soeller C
Journal of muscle research and cell motility 2016 Dec;37(6):195-202
Journal of muscle research and cell motility 2016 Dec;37(6):195-202
Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes.
Munro ML, Jayasinghe I, Wang Q, Quick A, Wang W, Baddeley D, Wehrens XH, Soeller C
Journal of cell science 2016 Dec 1;129(23):4388-4398
Journal of cell science 2016 Dec 1;129(23):4388-4398
Inhibition of Rac1 reduces store overload-induced calcium release and protects against ventricular arrhythmia.
Zhang L, Lu X, Gui L, Wu Y, Sims SM, Wang G, Feng Q
Journal of cellular and molecular medicine 2016 Aug;20(8):1513-22
Journal of cellular and molecular medicine 2016 Aug;20(8):1513-22
Store-operated calcium entry and the localization of STIM1 and Orai1 proteins in isolated mouse sinoatrial node cells.
Liu J, Xin L, Benson VL, Allen DG, Ju YK
Frontiers in physiology 2015;6:69
Frontiers in physiology 2015;6:69
Cellular Hypertrophy and Increased Susceptibility to Spontaneous Calcium-Release of Rat Left Atrial Myocytes Due to Elevated Afterload.
Zhang H, Cannell MB, Kim SJ, Watson JJ, Norman R, Calaghan SC, Orchard CH, James AF
PloS one 2015;10(12):e0144309
PloS one 2015;10(12):e0144309
Altered distribution of ICa impairs Ca release at the t-tubules of ventricular myocytes from failing hearts.
Bryant SM, Kong CH, Watson J, Cannell MB, James AF, Orchard CH
Journal of molecular and cellular cardiology 2015 Sep;86:23-31
Journal of molecular and cellular cardiology 2015 Sep;86:23-31
Examination of the Effects of Heterogeneous Organization of RyR Clusters, Myofibrils and Mitochondria on Ca2+ Release Patterns in Cardiomyocytes.
Rajagopal V, Bass G, Walker CG, Crossman DJ, Petzer A, Hickey A, Siekmann I, Hoshijima M, Ellisman MH, Crampin EJ, Soeller C
PLoS computational biology 2015 Sep;11(9):e1004417
PLoS computational biology 2015 Sep;11(9):e1004417
Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging.
Crossman DJ, Hou Y, Jayasinghe I, Baddeley D, Soeller C
Methods (San Diego, Calif.) 2015 Oct 15;88:98-108
Methods (San Diego, Calif.) 2015 Oct 15;88:98-108
Cardiac Resynchronization Therapy Reduces Subcellular Heterogeneity of Ryanodine Receptors, T-Tubules, and Ca2+ Sparks Produced by Dyssynchronous Heart Failure.
Li H, Lichter JG, Seidel T, Tomaselli GF, Bridge JH, Sachse FB
Circulation. Heart failure 2015 Nov;8(6):1105-14
Circulation. Heart failure 2015 Nov;8(6):1105-14
Excess TGF-β mediates muscle weakness associated with bone metastases in mice.
Waning DL, Mohammad KS, Reiken S, Xie W, Andersson DC, John S, Chiechi A, Wright LE, Umanskaya A, Niewolna M, Trivedi T, Charkhzarrin S, Khatiwada P, Wronska A, Haynes A, Benassi MS, Witzmann FA, Zhen G, Wang X, Cao X, Roodman GD, Marks AR, Guise TA
Nature medicine 2015 Nov;21(11):1262-1271
Nature medicine 2015 Nov;21(11):1262-1271
Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes.
Hou Y, Jayasinghe I, Crossman DJ, Baddeley D, Soeller C
Journal of molecular and cellular cardiology 2015 Mar;80:45-55
Journal of molecular and cellular cardiology 2015 Mar;80:45-55
RyR2 modulates a Ca2+-activated K+ current in mouse cardiac myocytes.
Mu YH, Zhao WC, Duan P, Chen Y, Zhao WD, Wang Q, Tu HY, Zhang Q
PloS one 2014;9(4):e94905
PloS one 2014;9(4):e94905
Hierarchical clustering of ryanodine receptors enables emergence of a calcium clock in sinoatrial node cells.
Stern MD, Maltseva LA, Juhaszova M, Sollott SJ, Lakatta EG, Maltsev VA
The Journal of general physiology 2014 May;143(5):577-604
The Journal of general physiology 2014 May;143(5):577-604
TRPV2 is critical for the maintenance of cardiac structure and function in mice.
Katanosaka Y, Iwasaki K, Ujihara Y, Takatsu S, Nishitsuji K, Kanagawa M, Sudo A, Toda T, Katanosaka K, Mohri S, Naruse K
Nature communications 2014 May 29;5:3932
Nature communications 2014 May 29;5:3932
Age-associated abnormalities of intrinsic automaticity of sinoatrial nodal cells are linked to deficient cAMP-PKA-Ca(2+) signaling.
Liu J, Sirenko S, Juhaszova M, Sollott SJ, Shukla S, Yaniv Y, Lakatta EG
American journal of physiology. Heart and circulatory physiology 2014 May 15;306(10):H1385-97
American journal of physiology. Heart and circulatory physiology 2014 May 15;306(10):H1385-97
Suppression of InsP3 receptor-mediated Ca2+ signaling alleviates mutant presenilin-linked familial Alzheimer's disease pathogenesis.
Shilling D, Müller M, Takano H, Mak DO, Abel T, Coulter DA, Foskett JK
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 May 14;34(20):6910-23
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 May 14;34(20):6910-23
A modified local control model for Ca2+ transients in cardiomyocytes: junctional flux is accompanied by release from adjacent non-junctional RyRs.
Torres NS, Sachse FB, Izu LT, Goldhaber JI, Spitzer KW, Bridge JH
Journal of molecular and cellular cardiology 2014 Mar;68:1-11
Journal of molecular and cellular cardiology 2014 Mar;68:1-11
Oxidation- and CaMKII-mediated sarcoplasmic reticulum Ca(2+) leak triggers atrial fibrillation in aging.
Guo X, Yuan S, Liu Z, Fang Q
Journal of cardiovascular electrophysiology 2014 Jun;25(6):645-52
Journal of cardiovascular electrophysiology 2014 Jun;25(6):645-52
Single delivery of an adeno-associated viral construct to transfer the CASQ2 gene to knock-in mice affected by catecholaminergic polymorphic ventricular tachycardia is able to cure the disease from birth to advanced age.
Denegri M, Bongianino R, Lodola F, Boncompagni S, De Giusti VC, Avelino-Cruz JE, Liu N, Persampieri S, Curcio A, Esposito F, Pietrangelo L, Marty I, Villani L, Moyaho A, Baiardi P, Auricchio A, Protasi F, Napolitano C, Priori SG
Circulation 2014 Jun 24;129(25):2673-81
Circulation 2014 Jun 24;129(25):2673-81
Early remodeling of perinuclear Ca2+ stores and nucleoplasmic Ca2+ signaling during the development of hypertrophy and heart failure.
Ljubojevic S, Radulovic S, Leitinger G, Sedej S, Sacherer M, Holzer M, Winkler C, Pritz E, Mittler T, Schmidt A, Sereinigg M, Wakula P, Zissimopoulos S, Bisping E, Post H, Marsche G, Bossuyt J, Bers DM, Kockskämper J, Pieske B
Circulation 2014 Jul 15;130(3):244-55
Circulation 2014 Jul 15;130(3):244-55
Loss of microRNA-106b-25 cluster promotes atrial fibrillation by enhancing ryanodine receptor type-2 expression and calcium release.
Chiang DY, Kongchan N, Beavers DL, Alsina KM, Voigt N, Neilson JR, Jakob H, Martin JF, Dobrev D, Wehrens XH, Li N
Circulation. Arrhythmia and electrophysiology 2014 Dec;7(6):1214-22
Circulation. Arrhythmia and electrophysiology 2014 Dec;7(6):1214-22
Reduced aerobic capacity causes leaky ryanodine receptors that trigger arrhythmia in a rat strain artificially selected and bred for low aerobic running capacity.
Høydal MA, Stølen TO, Johnsen AB, Alvez M, Catalucci D, Condorelli G, Koch LG, Britton SL, Smith GL, Wisløff U
Acta physiologica (Oxford, England) 2014 Apr;210(4):854-64
Acta physiologica (Oxford, England) 2014 Apr;210(4):854-64
Reduced IRE1α mediates apoptotic cell death by disrupting calcium homeostasis via the InsP3 receptor.
Son SM, Byun J, Roh SE, Kim SJ, Mook-Jung I
Cell death & disease 2014 Apr 17;5(4):e1188
Cell death & disease 2014 Apr 17;5(4):e1188
Cardio-protection by Ginkgo biloba extract 50 in rats with acute myocardial infarction is related to Na⁺-Ca²⁺ exchanger.
Liu AH, Bao YM, Wang XY, Zhang ZX
The American journal of Chinese medicine 2013;41(4):789-800
The American journal of Chinese medicine 2013;41(4):789-800
Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.
Yi T, Vick JS, Vecchio MJ, Begin KJ, Bell SP, Delay RJ, Palmer BM
American journal of physiology. Heart and circulatory physiology 2013 Sep 1;305(5):H706-15
American journal of physiology. Heart and circulatory physiology 2013 Sep 1;305(5):H706-15
Inhibition of the late sodium current slows t-tubule disruption during the progression of hypertensive heart disease in the rat.
Aistrup GL, Gupta DK, Kelly JE, O'Toole MJ, Nahhas A, Chirayil N, Misener S, Beussink L, Singh N, Ng J, Reddy M, Mongkolrattanothai T, El-Bizri N, Rajamani S, Shryock JC, Belardinelli L, Shah SJ, Wasserstrom JA
American journal of physiology. Heart and circulatory physiology 2013 Oct 1;305(7):H1068-79
American journal of physiology. Heart and circulatory physiology 2013 Oct 1;305(7):H1068-79
Cardiomyocyte ryanodine receptor degradation by chaperone-mediated autophagy.
Pedrozo Z, Torrealba N, Fernández C, Gatica D, Toro B, Quiroga C, Rodriguez AE, Sanchez G, Gillette TG, Hill JA, Donoso P, Lavandero S
Cardiovascular research 2013 May 1;98(2):277-85
Cardiovascular research 2013 May 1;98(2):277-85
Phospholipase Cε hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy.
Zhang L, Malik S, Pang J, Wang H, Park KM, Yule DI, Blaxall BC, Smrcka AV
Cell 2013 Mar 28;153(1):216-27
Cell 2013 Mar 28;153(1):216-27
Bradycardia alters Ca(2+) dynamics enhancing dispersion of repolarization and arrhythmia risk.
Kim JJ, Němec J, Papp R, Strongin R, Abramson JJ, Salama G
American journal of physiology. Heart and circulatory physiology 2013 Mar 15;304(6):H848-60
American journal of physiology. Heart and circulatory physiology 2013 Mar 15;304(6):H848-60
Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons.
Kaya L, Meissner B, Riedl MC, Muik M, Schwarzer C, Ferraguti F, Sarg B, Lindner H, Schweigreiter R, Knaus HG, Romanin C, Bandtlow CE
Biochimica et biophysica acta 2013 Jun;1833(6):1421-33
Biochimica et biophysica acta 2013 Jun;1833(6):1421-33
Impaired contractile function due to decreased cardiac myosin binding protein C content in the sarcomere.
Cheng Y, Wan X, McElfresh TA, Chen X, Gresham KS, Rosenbaum DS, Chandler MP, Stelzer JE
American journal of physiology. Heart and circulatory physiology 2013 Jul 1;305(1):H52-65
American journal of physiology. Heart and circulatory physiology 2013 Jul 1;305(1):H52-65
Large-conductance calcium-activated potassium current modulates excitability in isolated canine intracardiac neurons.
Pérez GJ, Desai M, Anderson S, Scornik FS
American journal of physiology. Cell physiology 2013 Feb 1;304(3):C280-6
American journal of physiology. Cell physiology 2013 Feb 1;304(3):C280-6
Ionic bases for electrical remodeling of the canine cardiac ventricle.
Jeyaraj D, Wan X, Ficker E, Stelzer JE, Deschenes I, Liu H, Wilson LD, Decker KF, Said TH, Jain MK, Rudy Y, Rosenbaum DS
American journal of physiology. Heart and circulatory physiology 2013 Aug 1;305(3):H410-9
American journal of physiology. Heart and circulatory physiology 2013 Aug 1;305(3):H410-9
Advanced glycation end product cross-link breaker attenuates diabetes-induced cardiac dysfunction by improving sarcoplasmic reticulum calcium handling.
Kranstuber AL, Del Rio C, Biesiadecki BJ, Hamlin RL, Ottobre J, Gyorke S, Lacombe VA
Frontiers in physiology 2012;3:292
Frontiers in physiology 2012;3:292
JTV519 (K201) reduces sarcoplasmic reticulum Ca²⁺ leak and improves diastolic function in vitro in murine and human non-failing myocardium.
Sacherer M, Sedej S, Wakuła P, Wallner M, Vos MA, Kockskämper J, Stiegler P, Sereinigg M, von Lewinski D, Antoons G, Pieske BM, Heinzel FR, CONTICA investigators
British journal of pharmacology 2012 Oct;167(3):493-504
British journal of pharmacology 2012 Oct;167(3):493-504
Targeted sarcoplasmic reticulum Ca2+ ATPase 2a gene delivery to restore electrical stability in the failing heart.
Cutler MJ, Wan X, Plummer BN, Liu H, Deschenes I, Laurita KR, Hajjar RJ, Rosenbaum DS
Circulation 2012 Oct 23;126(17):2095-104
Circulation 2012 Oct 23;126(17):2095-104
Zinc-induced cardiomyocyte relaxation in a rat model of hyperglycemia is independent of myosin isoform.
Yi T, Cheema Y, Tremble SM, Bell SP, Chen Z, Subramanian M, LeWinter MM, VanBuren P, Palmer BM
Cardiovascular diabetology 2012 Nov 2;11:135
Cardiovascular diabetology 2012 Nov 2;11:135
Coupling of ryanodine receptor 2 and voltage-dependent anion channel 2 is essential for Ca²+ transfer from the sarcoplasmic reticulum to the mitochondria in the heart.
Min CK, Yeom DR, Lee KE, Kwon HK, Kang M, Kim YS, Park ZY, Jeon H, Kim DH
The Biochemical journal 2012 Nov 1;447(3):371-9
The Biochemical journal 2012 Nov 1;447(3):371-9
Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Jung CB, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, Dorn T, Ruppenthal S, Pfeiffer S, Goedel A, Dirschinger RJ, Seyfarth M, Lam JT, Sinnecker D, Gudermann T, Lipp P, Laugwitz KL
EMBO molecular medicine 2012 Mar;4(3):180-91
EMBO molecular medicine 2012 Mar;4(3):180-91
Extreme sarcoplasmic reticulum volume loss and compensatory T-tubule remodeling after Serca2 knockout.
Swift F, Franzini-Armstrong C, Øyehaug L, Enger UH, Andersson KB, Christensen G, Sejersted OM, Louch WE
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 6;109(10):3997-4001
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 6;109(10):3997-4001
Amyloid peptide regulates calcium homoeostasis and arrhythmogenesis in pulmonary vein cardiomyocytes.
Tsao HM, Weerateerangkul P, Chen YC, Kao YH, Lin YK, Huang JH, Chen SA, Chen YJ
European journal of clinical investigation 2012 Jun;42(6):589-98
European journal of clinical investigation 2012 Jun;42(6):589-98
Probenecid: novel use as a non-injurious positive inotrope acting via cardiac TRPV2 stimulation.
Koch SE, Gao X, Haar L, Jiang M, Lasko VM, Robbins N, Cai W, Brokamp C, Varma P, Tranter M, Liu Y, Ren X, Lorenz JN, Wang HS, Jones WK, Rubinstein J
Journal of molecular and cellular cardiology 2012 Jul;53(1):134-44
Journal of molecular and cellular cardiology 2012 Jul;53(1):134-44
Overexpression of histidine-rich calcium binding protein in equine ventricular myocardium.
Sacchetto R, Sharova E, Patruno M, Maccatrozzo L, Damiani E, Mascarello F
Veterinary journal (London, England : 1997) 2012 Jul;193(1):157-61
Veterinary journal (London, England : 1997) 2012 Jul;193(1):157-61
Combined insulin treatment and intense exercise training improved basal cardiac function and Ca(2+)-cycling proteins expression in type 1 diabetic rats.
Le Douairon Lahaye S, Gratas-Delamarche A, Malardé L, Zguira S, Vincent S, Lemoine Morel S, Carré F, Rannou Bekono F
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2012 Feb;37(1):53-62
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme 2012 Feb;37(1):53-62
ACE inhibition prevents diastolic Ca2+ overload and loss of myofilament Ca2+ sensitivity after myocardial infarction.
Zalvidea S, André L, Loyer X, Cassan C, Sainte-Marie Y, Thireau J, Sjaastad I, Heymes C, Pasquié JL, Cazorla O, Aimond F, Richard S
Current molecular medicine 2012 Feb;12(2):206-17
Current molecular medicine 2012 Feb;12(2):206-17
Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.
Wagner E, Lauterbach MA, Kohl T, Westphal V, Williams GS, Steinbrecher JH, Streich JH, Korff B, Tuan HT, Hagen B, Luther S, Hasenfuss G, Parlitz U, Jafri MS, Hell SW, Lederer WJ, Lehnart SE
Circulation research 2012 Aug 3;111(4):402-14
Circulation research 2012 Aug 3;111(4):402-14
Ca²+-regulatory proteins in cardiomyocytes from the right ventricle in children with congenital heart disease.
Wu Y, Feng W, Zhang H, Li S, Wang D, Pan X, Hu S
Journal of translational medicine 2012 Apr 2;10:67
Journal of translational medicine 2012 Apr 2;10:67
4D super-resolution microscopy with conventional fluorophores and single wavelength excitation in optically thick cells and tissues.
Baddeley D, Crossman D, Rossberger S, Cheyne JE, Montgomery JM, Jayasinghe ID, Cremer C, Cannell MB, Soeller C
PloS one 2011;6(5):e20645
PloS one 2011;6(5):e20645
Cardiac calcium pump inactivation and nitrosylation in senescent rat myocardium are not attenuated by long-term treadmill training.
Thomas MM, Vigna C, Betik AC, Tupling AR, Hepple RT
Experimental gerontology 2011 Oct;46(10):803-10
Experimental gerontology 2011 Oct;46(10):803-10
FKBP12.6 mice display temporal gender differences in cardiac Ca(2+)-signalling phenotype upon chronic pressure overload.
Prévilon M, Pezet M, Semprez F, Mercadier JJ, Rouet-Benzineb P
Canadian journal of physiology and pharmacology 2011 Nov;89(11):769-82
Canadian journal of physiology and pharmacology 2011 Nov;89(11):769-82
Ca(2+)-related signaling and protein phosphorylation abnormalities play central roles in a new experimental model of electrical storm.
Tsuji Y, Hojo M, Voigt N, El-Armouche A, Inden Y, Murohara T, Dobrev D, Nattel S, Kodama I, Kamiya K
Circulation 2011 May 24;123(20):2192-203
Circulation 2011 May 24;123(20):2192-203
Changes in the organization of excitation-contraction coupling structures in failing human heart.
Crossman DJ, Ruygrok PN, Soeller C, Cannell MB
PloS one 2011 Mar 9;6(3):e17901
PloS one 2011 Mar 9;6(3):e17901
Exercise training does not improve cardiac function in compensated or decompensated left ventricular hypertrophy induced by aortic stenosis.
van Deel ED, de Boer M, Kuster DW, Boontje NM, Holemans P, Sipido KR, van der Velden J, Duncker DJ
Journal of molecular and cellular cardiology 2011 Jun;50(6):1017-25
Journal of molecular and cellular cardiology 2011 Jun;50(6):1017-25
Loss of the AE3 anion exchanger in a hypertrophic cardiomyopathy model causes rapid decompensation and heart failure.
Al Moamen NJ, Prasad V, Bodi I, Miller ML, Neiman ML, Lasko VM, Alper SL, Wieczorek DF, Lorenz JN, Shull GE
Journal of molecular and cellular cardiology 2011 Jan;50(1):137-46
Journal of molecular and cellular cardiology 2011 Jan;50(1):137-46
Effects of exercise training combined with insulin treatment on cardiac NOS1 signaling pathways in type 1 diabetic rats.
Le Douairon Lahaye S, Rebillard A, Zguira MS, Malardé L, Saïag B, Gratas-Delamarche A, Carré F, Bekono FR
Molecular and cellular biochemistry 2011 Jan;347(1-2):53-62
Molecular and cellular biochemistry 2011 Jan;347(1-2):53-62
Reduced Ca2+ spark activity after subarachnoid hemorrhage disables BK channel control of cerebral artery tone.
Koide M, Nystoriak MA, Krishnamoorthy G, O'Connor KP, Bonev AD, Nelson MT, Wellman GC
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2011 Jan;31(1):3-16
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2011 Jan;31(1):3-16
Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity and spatial memory formation.
Adasme T, Haeger P, Paula-Lima AC, Espinoza I, Casas-Alarcón MM, Carrasco MA, Hidalgo C
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 15;108(7):3029-34
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 15;108(7):3029-34
Couplons in rat atria form distinct subgroups defined by their molecular partners.
Schulson MN, Scriven DR, Fletcher P, Moore ED
Journal of cell science 2011 Apr 1;124(Pt 7):1167-74
Journal of cell science 2011 Apr 1;124(Pt 7):1167-74
Multi-image colocalization and its statistical significance.
Fletcher PA, Scriven DR, Schulson MN, Moore ED
Biophysical journal 2010 Sep 22;99(6):1996-2005
Biophysical journal 2010 Sep 22;99(6):1996-2005
Sequential alterations in Akt, GSK3β, and calcineurin signalling in the mouse left ventricle after thoracic aortic constriction.
Prévilon M, Pezet M, Dachez C, Mercadier JJ, Rouet-Benzineb P
Canadian journal of physiology and pharmacology 2010 Nov;88(11):1093-101
Canadian journal of physiology and pharmacology 2010 Nov;88(11):1093-101
A new twist in cardiac muscle: dislocated and helicoid arrangements of myofibrillar z-disks in mammalian ventricular myocytes.
Jayasinghe ID, Crossman DJ, Soeller C, Cannell MB
Journal of molecular and cellular cardiology 2010 May;48(5):964-71
Journal of molecular and cellular cardiology 2010 May;48(5):964-71
Chronic heart rate reduction with ivabradine improves systolic function of the reperfused heart through a dual mechanism involving a direct mechanical effect and a long-term increase in FKBP12/12.6 expression.
Couvreur N, Tissier R, Pons S, Chetboul V, Gouni V, Bruneval P, Mandet C, Pouchelon JL, Berdeaux A, Ghaleh B
European heart journal 2010 Jun;31(12):1529-37
European heart journal 2010 Jun;31(12):1529-37
A novel ryanodine receptor expressed in pancreatic islets by alternative splicing from type 2 ryanodine receptor gene.
Takasawa S, Kuroki M, Nata K, Noguchi N, Ikeda T, Yamauchi A, Ota H, Itaya-Hironaka A, Sakuramoto-Tsuchida S, Takahashi I, Yoshikawa T, Shimosegawa T, Okamoto H
Biochemical and biophysical research communications 2010 Jun 25;397(2):140-5
Biochemical and biophysical research communications 2010 Jun 25;397(2):140-5
Succinate modulates Ca(2+) transient and cardiomyocyte viability through PKA-dependent pathway.
Aguiar CJ, Andrade VL, Gomes ER, Alves MN, Ladeira MS, Pinheiro AC, Gomes DA, Almeida AP, Goes AM, Resende RR, Guatimosim S, Leite MF
Cell calcium 2010 Jan;47(1):37-46
Cell calcium 2010 Jan;47(1):37-46
Phospholamban ablation rescues sarcoplasmic reticulum Ca(2+) handling but exacerbates cardiac dysfunction in CaMKIIdelta(C) transgenic mice.
Zhang T, Guo T, Mishra S, Dalton ND, Kranias EG, Peterson KL, Bers DM, Brown JH
Circulation research 2010 Feb 5;106(2):354-62
Circulation research 2010 Feb 5;106(2):354-62
Ryanodine receptor phosphorylation by calcium/calmodulin-dependent protein kinase II promotes life-threatening ventricular arrhythmias in mice with heart failure.
van Oort RJ, McCauley MD, Dixit SS, Pereira L, Yang Y, Respress JL, Wang Q, De Almeida AC, Skapura DG, Anderson ME, Bers DM, Wehrens XH
Circulation 2010 Dec 21;122(25):2669-79
Circulation 2010 Dec 21;122(25):2669-79
Regulation of myocyte contraction via neuronal nitric oxide synthase: role of ryanodine receptor S-nitrosylation.
Wang H, Viatchenko-Karpinski S, Sun J, Györke I, Benkusky NA, Kohr MJ, Valdivia HH, Murphy E, Györke S, Ziolo MT
The Journal of physiology 2010 Aug 1;588(Pt 15):2905-17
The Journal of physiology 2010 Aug 1;588(Pt 15):2905-17
Defective calmodulin binding to the cardiac ryanodine receptor plays a key role in CPVT-associated channel dysfunction.
Xu X, Yano M, Uchinoumi H, Hino A, Suetomi T, Ono M, Tateishi H, Oda T, Okuda S, Doi M, Kobayashi S, Yamamoto T, Ikeda Y, Ikemoto N, Matsuzaki M
Biochemical and biophysical research communications 2010 Apr 9;394(3):660-6
Biochemical and biophysical research communications 2010 Apr 9;394(3):660-6
Distribution of ryanodine receptors in rat ventricular myocytes.
Salnikov V, Lukyanenko YO, Lederer WJ, Lukyanenko V
Journal of muscle research and cell motility 2009;30(3-4):161-70
Journal of muscle research and cell motility 2009;30(3-4):161-70
Three-dimensional high-resolution imaging of cardiac proteins to construct models of intracellular Ca2+ signalling in rat ventricular myocytes.
Soeller C, Jayasinghe ID, Li P, Holden AV, Cannell MB
Experimental physiology 2009 May;94(5):496-508
Experimental physiology 2009 May;94(5):496-508
Electrophysiological consequences of dyssynchronous heart failure and its restoration by resynchronization therapy.
Aiba T, Hesketh GG, Barth AS, Liu T, Daya S, Chakir K, Dimaano VL, Abraham TP, O'Rourke B, Akar FG, Kass DA, Tomaselli GF
Circulation 2009 Mar 10;119(9):1220-30
Circulation 2009 Mar 10;119(9):1220-30
Increased Ca2+ sensitivity of the ryanodine receptor mutant RyR2R4496C underlies catecholaminergic polymorphic ventricular tachycardia.
Fernández-Velasco M, Rueda A, Rizzi N, Benitah JP, Colombi B, Napolitano C, Priori SG, Richard S, Gómez AM
Circulation research 2009 Jan 30;104(2):201-9, 12p following 209
Circulation research 2009 Jan 30;104(2):201-9, 12p following 209
The unliganded long isoform of estrogen receptor beta stimulates brain ryanodine receptor single channel activity alongside with cytosolic Ca2+.
Rybalchenko V, Grillo MA, Gastinger MJ, Rybalchenko N, Payne AJ, Koulen P
Journal of receptor and signal transduction research 2009 Dec;29(6):326-41
Journal of receptor and signal transduction research 2009 Dec;29(6):326-41
Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes.
Baddeley D, Jayasinghe I, Lam L, Rossberger S, Cannell MB, Soeller C
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 29;106(52):22275-80
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 29;106(52):22275-80
Induced overexpression of Na+/Ca2+ exchanger transgene: altered myocyte contractility, [Ca2+]i transients, SR Ca2+ contents, and action potential duration.
Wang J, Chan TO, Zhang XQ, Gao E, Song J, Koch WJ, Feldman AM, Cheung JY
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H590-601
American journal of physiology. Heart and circulatory physiology 2009 Aug;297(2):H590-601
Exercise training during diabetes attenuates cardiac ryanodine receptor dysregulation.
Shao CH, Wehrens XH, Wyatt TA, Parbhu S, Rozanski GJ, Patel KP, Bidasee KR
Journal of applied physiology (Bethesda, Md. : 1985) 2009 Apr;106(4):1280-92
Journal of applied physiology (Bethesda, Md. : 1985) 2009 Apr;106(4):1280-92
Physical coupling supports the local Ca2+ transfer between sarcoplasmic reticulum subdomains and the mitochondria in heart muscle.
García-Pérez C, Hajnóczky G, Csordás G
The Journal of biological chemistry 2008 Nov 21;283(47):32771-80
The Journal of biological chemistry 2008 Nov 21;283(47):32771-80
Differential alterations in expressions of ryanodine receptor subtypes in cerebellar cortical neurons of an ataxic mutant, rolling mouse Nagoya.
Sawada K, Hosoi E, Bando M, Sakata-Haga H, Lee NS, Jeong YG, Fukui Y
Neuroscience 2008 Mar 27;152(3):609-17
Neuroscience 2008 Mar 27;152(3):609-17
IP3 receptor-dependent Ca2+ release modulates excitation-contraction coupling in rabbit ventricular myocytes.
Domeier TL, Zima AV, Maxwell JT, Huke S, Mignery GA, Blatter LA
American journal of physiology. Heart and circulatory physiology 2008 Feb;294(2):H596-604
American journal of physiology. Heart and circulatory physiology 2008 Feb;294(2):H596-604
Wide long lasting perinuclear Ca2+ release events generated by an interaction between ryanodine and IP3 receptors in canine Purkinje cells.
Hirose M, Stuyvers B, Dun W, Ter Keurs H, Boyden PA
Journal of molecular and cellular cardiology 2008 Aug;45(2):176-84
Journal of molecular and cellular cardiology 2008 Aug;45(2):176-84
Unexpected structural and functional consequences of the R33Q homozygous mutation in cardiac calsequestrin: a complex arrhythmogenic cascade in a knock in mouse model.
Rizzi N, Liu N, Napolitano C, Nori A, Turcato F, Colombi B, Bicciato S, Arcelli D, Spedito A, Scelsi M, Villani L, Esposito G, Boncompagni S, Protasi F, Volpe P, Priori SG
Circulation research 2008 Aug 1;103(3):298-306
Circulation research 2008 Aug 1;103(3):298-306
Three-dimensional distribution of cardiac Na+-Ca2+ exchanger and ryanodine receptor during development.
Dan P, Lin E, Huang J, Biln P, Tibbits GF
Biophysical journal 2007 Oct 1;93(7):2504-18
Biophysical journal 2007 Oct 1;93(7):2504-18
Cellular and molecular determinants of altered Ca2+ handling in the failing rabbit heart: primary defects in SR Ca2+ uptake and release mechanisms.
Armoundas AA, Rose J, Aggarwal R, Stuyvers BD, O'rourke B, Kass DA, Marbán E, Shorofsky SR, Tomaselli GF, William Balke C
American journal of physiology. Heart and circulatory physiology 2007 Mar;292(3):H1607-18
American journal of physiology. Heart and circulatory physiology 2007 Mar;292(3):H1607-18
CaMKII-mediated increased lusitropic responses to beta-adrenoreceptor stimulation in ANP-receptor deficient mice.
Yurukova S, Kilić A, Völker K, Leineweber K, Dybkova N, Maier LS, Brodde OE, Kuhn M
Cardiovascular research 2007 Mar 1;73(4):678-88
Cardiovascular research 2007 Mar 1;73(4):678-88
Thermogenic activity of Ca2+-ATPase from skeletal muscle heavy sarcoplasmic reticulum: the role of ryanodine Ca2+ channel.
Arruda AP, Nigro M, Oliveira GM, de Meis L
Biochimica et biophysica acta 2007 Jun;1768(6):1498-505
Biochimica et biophysica acta 2007 Jun;1768(6):1498-505
Retina expresses a novel variant of the ryanodine receptor.
Shoshan-Barmatz V, Zakar M, Shmuelivich F, Nahon E, Vardi N
The European journal of neuroscience 2007 Dec;26(11):3113-25
The European journal of neuroscience 2007 Dec;26(11):3113-25
Identification and functional distribution of intracellular ca channels in mouse lacrimal gland acinar cells.
Medina-Ortiz WE, Gregg EV, Brun-Zinkernagel AM, Koulen P
The open ophthalmology journal 2007 Dec 4;1:8-16
The open ophthalmology journal 2007 Dec 4;1:8-16
Distribution of inositol-1,4,5-trisphosphate receptor isotypes and ryanodine receptor isotypes during maturation of the rat hippocampus.
Hertle DN, Yeckel MF
Neuroscience 2007 Dec 12;150(3):625-38
Neuroscience 2007 Dec 12;150(3):625-38
Functional groups of ryanodine receptors in rat ventricular cells.
Lukyanenko V, Ziman A, Lukyanenko A, Salnikov V, Lederer WJ
The Journal of physiology 2007 Aug 15;583(Pt 1):251-69
The Journal of physiology 2007 Aug 15;583(Pt 1):251-69
Functional uncoupling between Ca2+ release and afterhyperpolarization in mutant hippocampal neurons lacking junctophilins.
Moriguchi S, Nishi M, Komazaki S, Sakagami H, Miyazaki T, Masumiya H, Saito SY, Watanabe M, Kondo H, Yawo H, Fukunaga K, Takeshima H
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 11;103(28):10811-6
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 11;103(28):10811-6
New cardioprotective agent K201 is natriuretic and glomerular filtration rate enhancing.
Lisy O, Burnett JC Jr
Circulation 2006 Jan 17;113(2):246-51
Circulation 2006 Jan 17;113(2):246-51
Increased sarcoplasmic reticulum calcium leak but unaltered contractility by acute CaMKII overexpression in isolated rabbit cardiac myocytes.
Kohlhaas M, Zhang T, Seidler T, Zibrova D, Dybkova N, Steen A, Wagner S, Chen L, Brown JH, Bers DM, Maier LS
Circulation research 2006 Feb 3;98(2):235-44
Circulation research 2006 Feb 3;98(2):235-44
Increased susceptibility to isoproterenol-induced cardiac hypertrophy and impaired weight gain in mice lacking the histidine-rich calcium-binding protein.
Jaehnig EJ, Heidt AB, Greene SB, Cornelissen I, Black BL
Molecular and cellular biology 2006 Dec;26(24):9315-26
Molecular and cellular biology 2006 Dec;26(24):9315-26
Caveolin-3 is adjacent to a group of extradyadic ryanodine receptors.
Scriven DR, Klimek A, Asghari P, Bellve K, Moore ED
Biophysical journal 2005 Sep;89(3):1893-901
Biophysical journal 2005 Sep;89(3):1893-901
Ca2+ sparks and waves in canine purkinje cells: a triple layered system of Ca2+ activation.
Stuyvers BD, Dun W, Matkovich S, Sorrentino V, Boyden PA, ter Keurs HE
Circulation research 2005 Jul 8;97(1):35-43
Circulation research 2005 Jul 8;97(1):35-43
Distinct subcellular location of the Ca2+-binding protein S100A1 differentially modulates Ca2+-cycling in ventricular rat cardiomyocytes.
Most P, Boerries M, Eicher C, Schweda C, Völkers M, Wedel T, Söllner S, Katus HA, Remppis A, Aebi U, Koch WJ, Schoenenberger CA
Journal of cell science 2005 Jan 15;118(Pt 2):421-31
Journal of cell science 2005 Jan 15;118(Pt 2):421-31
Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes.
Terentyev D, Cala SE, Houle TD, Viatchenko-Karpinski S, Gyorke I, Terentyeva R, Williams SC, Gyorke S
Circulation research 2005 Apr 1;96(6):651-8
Circulation research 2005 Apr 1;96(6):651-8
RyR2 and calpain-10 delineate a novel apoptosis pathway in pancreatic islets.
Johnson JD, Han Z, Otani K, Ye H, Zhang Y, Wu H, Horikawa Y, Misler S, Bell GI, Polonsky KS
The Journal of biological chemistry 2004 Jun 4;279(23):24794-802
The Journal of biological chemistry 2004 Jun 4;279(23):24794-802
Calcium/calmodulin-dependent protein kinase IIdelta associates with the ryanodine receptor complex and regulates channel function in rabbit heart.
Currie S, Loughrey CM, Craig MA, Smith GL
The Biochemical journal 2004 Jan 15;377(Pt 2):357-66
The Biochemical journal 2004 Jan 15;377(Pt 2):357-66
Dilated cardiomyopathy caused by tissue-specific ablation of SC35 in the heart.
Ding JH, Xu X, Yang D, Chu PH, Dalton ND, Ye Z, Yeakley JM, Cheng H, Xiao RP, Ross J, Chen J, Fu XD
The EMBO journal 2004 Feb 25;23(4):885-96
The EMBO journal 2004 Feb 25;23(4):885-96
Identification of biochemical adaptations in hyper- or hypocontractile hearts from phospholamban mutant mice by expression proteomics.
Pan Y, Kislinger T, Gramolini AO, Zvaritch E, Kranias EG, MacLennan DH, Emili A
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 24;101(8):2241-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 24;101(8):2241-6
ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors.
Zhang WM, Yip KP, Lin MJ, Shimoda LA, Li WH, Sham JS
American journal of physiology. Lung cellular and molecular physiology 2003 Sep;285(3):L680-90
American journal of physiology. Lung cellular and molecular physiology 2003 Sep;285(3):L680-90
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation.
Xu KY, Kuppusamy SP, Wang JQ, Li H, Cui H, Dawson TM, Huang PL, Burnett AL, Kuppusamy P, Becker LC
The Journal of biological chemistry 2003 Oct 24;278(43):41798-803
The Journal of biological chemistry 2003 Oct 24;278(43):41798-803
Ca2+ regulatory systems in rat myocardium are altered by 24 weeks treadmill training.
Morán M, Saborido A, Megías A
Pflugers Archiv : European journal of physiology 2003 May;446(2):161-8
Pflugers Archiv : European journal of physiology 2003 May;446(2):161-8
Effect of tachycardia on myocardial sarcoplasmic reticulum and Ca2+ dynamics: a mechanism for preconditioning?
Domenech RJ, Sánchez G, Donoso P, Parra V, Macho P
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Journal of molecular and cellular cardiology 2003 Dec;35(12):1429-37
Spatial and temporal inhomogeneities during Ca2+ release from the sarcoplasmic reticulum in pig ventricular myocytes.
Heinzel FR, Bito V, Volders PG, Antoons G, Mubagwa K, Sipido KR
Circulation research 2002 Nov 29;91(11):1023-30
Circulation research 2002 Nov 29;91(11):1023-30
The ryanodine receptor modulates the spontaneous beating rate of cardiomyocytes during development.
Yang HT, Tweedie D, Wang S, Guia A, Vinogradova T, Bogdanov K, Allen PD, Stern MD, Lakatta EG, Boheler KR
Proceedings of the National Academy of Sciences of the United States of America 2002 Jul 9;99(14):9225-30
Proceedings of the National Academy of Sciences of the United States of America 2002 Jul 9;99(14):9225-30
Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling.
Bruce JI, Shuttleworth TJ, Giovannucci DR, Yule DI
The Journal of biological chemistry 2002 Jan 11;277(2):1340-8
The Journal of biological chemistry 2002 Jan 11;277(2):1340-8
Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons.
Antonucci DE, Lim ST, Vassanelli S, Trimmer JS
Neuroscience 2001;108(1):69-81
Neuroscience 2001;108(1):69-81
cADP-ribose/ryanodine channel/Ca2+-release signal transduction pathway in mesangial cells.
Yusufi AN, Cheng J, Thompson MA, Dousa TP, Warner GM, Walker HJ, Grande JP
American journal of physiology. Renal physiology 2001 Jul;281(1):F91-F102
American journal of physiology. Renal physiology 2001 Jul;281(1):F91-F102
Ryanodine receptor dysfunction in hearts of streptozotocin-induced diabetic rats.
Bidasee KR, Dinçer UD, Besch HR Jr
Molecular pharmacology 2001 Dec;60(6):1356-64
Molecular pharmacology 2001 Dec;60(6):1356-64
Regulation of aquaporin-2 trafficking by vasopressin in the renal collecting duct. Roles of ryanodine-sensitive Ca2+ stores and calmodulin.
Chou CL, Yip KP, Michea L, Kador K, Ferraris JD, Wade JB, Knepper MA
The Journal of biological chemistry 2000 Nov 24;275(47):36839-46
The Journal of biological chemistry 2000 Nov 24;275(47):36839-46
Intracellular Ca(2+) handling in vascular smooth muscle cells is affected by proliferation.
Vallot O, Combettes L, Jourdon P, Inamo J, Marty I, Claret M, Lompré AM
Arteriosclerosis, thrombosis, and vascular biology 2000 May;20(5):1225-35
Arteriosclerosis, thrombosis, and vascular biology 2000 May;20(5):1225-35
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
Froemming GR, Murray BE, Harmon S, Pette D, Ohlendieck K
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Biochimica et biophysica acta 2000 Jun 1;1466(1-2):151-68
Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum.
Yamada KA, Kanter EM, Newatia A
Journal of cardiovascular pharmacology 2000 Jul;36(1):14-21
Journal of cardiovascular pharmacology 2000 Jul;36(1):14-21
Reversible block of the calcium release channel/ryanodine receptor by protamine, a heparin antidote.
Koulen P, Ehrlich BE
Molecular biology of the cell 2000 Jul;11(7):2213-9
Molecular biology of the cell 2000 Jul;11(7):2213-9
Expression and functional characterization of the cardiac muscle ryanodine receptor Ca(2+) release channel in Chinese hamster ovary cells.
Bhat MB, Hayek SM, Zhao J, Zang W, Takeshima H, Wier WG, Ma J
Biophysical journal 1999 Aug;77(2):808-16
Biophysical journal 1999 Aug;77(2):808-16
Ontogeny of local sarcoplasmic reticulum Ca2+ signals in cerebral arteries: Ca2+ sparks as elementary physiological events.
Gollasch M, Wellman GC, Knot HJ, Jaggar JH, Damon DH, Bonev AD, Nelson MT
Circulation research 1998 Nov 30;83(11):1104-14
Circulation research 1998 Nov 30;83(11):1104-14
A-kinase anchoring protein 100 (AKAP100) is localized in multiple subcellular compartments in the adult rat heart.
Yang J, Drazba JA, Ferguson DG, Bond M
The Journal of cell biology 1998 Jul 27;142(2):511-22
The Journal of cell biology 1998 Jul 27;142(2):511-22
Detection and functional characterization of ryanodine receptors from sea urchin eggs.
Lokuta AJ, Darszon A, Beltrán C, Valdivia HH
The Journal of physiology 1998 Jul 1;510 ( Pt 1)(Pt 1):155-64
The Journal of physiology 1998 Jul 1;510 ( Pt 1)(Pt 1):155-64
Characterization of recombinant rabbit cardiac and skeletal muscle Ca2+ release channels (ryanodine receptors) with a novel [3H]ryanodine binding assay.
Du GG, Imredy JP, MacLennan DH
The Journal of biological chemistry 1998 Dec 11;273(50):33259-66
The Journal of biological chemistry 1998 Dec 11;273(50):33259-66
Distribution of inositol-1,4,5-trisphosphate and ryanodine receptors in rat neostriatum.
Martone ME, Alba SA, Edelman VM, Airey JA, Ellisman MH
Brain research 1997 May 9;756(1-2):9-21
Brain research 1997 May 9;756(1-2):9-21
Compensatory mechanisms associated with the hyperdynamic function of phospholamban-deficient mouse hearts.
Chu G, Luo W, Slack JP, Tilgmann C, Sweet WE, Spindler M, Saupe KW, Boivin GP, Moravec CS, Matlib MA, Grupp IL, Ingwall JS, Kranias EG
Circulation research 1996 Dec;79(6):1064-76
Circulation research 1996 Dec;79(6):1064-76
Expression of a cardiac Ca(2+)-release channel isoform in mammalian brain.
Lai FA, Dent M, Wickenden C, Xu L, Kumari G, Misra M, Lee HB, Sar M, Meissner G
The Biochemical journal 1992 Dec 1;288 ( Pt 2)(Pt 2):553-64
The Biochemical journal 1992 Dec 1;288 ( Pt 2)(Pt 2):553-64
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Ryanodine Receptor Monoclonal Antibody (C3-33) (Product # MA3-916) and a 565 kDa band corresponding to Ryanodine receptor 1 was observed along with two uncharacterized band (*) at ~70 kDa and 100 kDa across tissues tested. Tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Mouse Colon (Lane 2) and Mouse Lung (Lane 3) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of rat brain hippocampus tissue shows that Ryanodine Receptor Monoclonal Antibody (Product # MA3-916) crossreacts with RyR1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2--figure supplement 2. Analysis of RNA and protein expression levels of the major cardiac Ca 2+ -handling proteins in PLN/DWORF Tg mice. ( A ) RNA levels of the indicated genes as quantified by qRT-PCR in 16-week-old heart tissue. Atp2a2 , SERCA2a; Ryr2 , ryanodine receptor 2; Cacna1c, alpha1C-subunit of the L-type Ca 2+ channel; Casq2 , calsequestrin 2. Data are normalized to 18S and presented as expression level relative to WT, mean +-SD for n = 10 mice per genotype. ( B ) Representative immunoblots of cardiac homogenates from mice with the indicated genotypes. PS16, phospho-serine 16 on PLN; PT17, phospho-threonine 17 on PLN; tPLN, total phospholamban; RyR2, ryanodine receptor 2; LTCC, L-type Ca 2+ channel (alpha1C-subunit); Casq2, calsequestrin 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ( C ) Western blots for n = 9-10 mice of each genotype were quantified using ImageJ and data are normalized to GAPDH and expressed as mean +-SD relative to WT. ( D ) Quantification of total phospholamban and its phosphorylation status as assessed by western blot ( B ) and expressed as relative to WT. Western blots were quantified with ImageJ software. Phosphorylation blots (PS16 and PT17) were normalized to total PLN (tPLN). Total PLN was normalized to GAPDH. Data are expressed as mean +-SD for n = 9-10 mice per genotype. Statistical comparisons between groups were evaluated by Student's t-test. p-value *p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6--figure supplement 1. Expression and post-translational modifications of Ca 2+ -handling proteins. ( A ) Immunoblots of cardiac homogenates from mice with the indicated genotypes. ( B ) Immunoblots were quantified using ImageJ and normalized to GAPDH. LTCC, L-type Ca 2+ channel (alpha1C-subunit); Casq2, calsequestrin 2; RyR2, ryanodine receptor 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. ( C ) Quantification of the phosphorylation status and oligomerization of phospholamban as assessed by western blot (panel A ) and expressed as relative to WT. Western blots were quantified with ImageJ software. Phosphorylation blots (PS16 and PT17) were normalized to total PLN (tPLN). Total PLN was normalized to GAPDH. tPLN, total phospholamban; PS16, phospho-serine 16 on PLN; PT17, phospho-threonine 17 on PLN. Data are expressed as mean +-SD for n = 4-6 mice per genotype. ( D ) RNA levels of the indicated genes as quantified by qRT-PCR in 8-week-old heart tissue. Atp2a2 , SERCA2a; Cacna1c, alpha1C-subunit of the L-type Ca 2+ channel; Casq2 , calsequestrin 2; Pln , phosholamban; Ryr2 , ryanodine receptor 2. Data are normalized to 18S values and presented as expression level relative to WT, mean +-SD for n = 4-5 mice per genotype.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Correlative confocal and super-resolution imaging of a human cardiac tissue section. The section was ~10 um thick and was labeled with phalloidin for f-Actin (Alexa 488), WGA for the cell membrane and extracellular matrix (Alexa 594), along with antibodies for the ryanodine receptor (RyR, Alexa 680) and calsequestrin (CSQ, Alexa 750). In addition to the applied labelling, a strong endogenous fluorescence signal from lipofuscin was recorded. The shorter wavelength labels (Actin, WGA, and RyR) were imaged on a confocal microscope, and the sample then taken to the localisation microscope where super-resolution imaging of the longer wavelength labels (RyR, lipofuscin, CSQ) was performed. Panel A shows an overview of the cellular structure across a large tissue area that is indicated by the actin labeling (largely muscle cell contractile protein). Scale bar 100 um. Panel B is a projection of a confocal stack taken of the region indicated in A. Scale bar 10 um. Panel C shows a confocal stack of a small detail area from B and panel D shows an optically sectioned super-resolution stack, within the region covered by the confocal stack in C. Panels E & F compare corresponding confocal (F) and super-resolution (E) images both using the RyR-Alexa 647 signal. Note the good correlation between the data and the improvement in resolution in E. Scale bar 1 um. G : 3-colour super-resolution image of a small area in the tissue sample, note the improved resolution as compared to the con
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Expression of Ca 2+ handling protein by isolated cardiomyocytes and electron micrographs of myofibrils in TRPV2-deficient hearts. ( a ) Time-dependent changes in the localization of the Ca 2+ regulatory proteins LTCC, RyR and NCX1 in tamoxifen-treated TRPV2 flox/flox ;Cre +/ - mice. Double staining with anti-LTCC antibody (green) and DAPI (blue) in cardiac muscle (left panels). Double staining with anti-RyR antibody (green) and DAPI (blue) in cardiac muscle (middle panels). Double staining with anti-NCX1 antibody (green) and DAPI (blue) in cardiomyocytes (right panels). Scale bar, 100 mum. ( b ) Expression of NCX1, LTCC, RyR and SERCA proteins in TRPV2-deficient heart extracts (10 mug per lane), using GAPDH as the internal control in cardiac muscle, (see full blots in Supplementary Fig. 6 ). ( c ) Electron micrographs of myofibrils in cardiomyocytes from TRPV2 flox/flox ;Cre +/ - mice, untreated or treated with tamoxifen. Scale bar, 5 mum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 6 Immunoblotting. Immunoblot analysis of key Ca 2+ handling proteins and phosphorylation was performed on tissue from left ventricles. SERCA2A abundance (A). PLB abundance (B) and phosphorylation on SER16 (C) and THR17 (D). NCX abundance (E). RyR abundance (F) with phosphorylation on SER2808 (G) and SER2814 (H). CaMKII abundance (I) and CaMKII phosphorylation on THR286 (J). PP1 (K) and PP2A (L) abundance. n heart = 6 for all analysis. *p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Notch Inhibition Cooperates with AKT1 to Enhance Cardiac Reprogramming alphaMHC-GFP MEFs (or GCaMP MEFs in C) were infected with GHMT or AGHMT, and treated with DMSO (vehicle) or DAPT. (A) Representative immunostaining images of GFP, alpha-actinin, and cTnT at day 15 of reprogramming. Scale bar, 200 mum. (B) Quantification of cells positive for alpha-actinin and cTnT as determined by immunostaining; n = 3 biological replicates. (C) Quantification of Ca 2+ flux-positive cells in GCaMP MEFs at day 15; n = 3 biological replicates. (D) Percentage of beating cells, relative to the number of input cells; n = 3 biological replicates. (E) Immunoblot against the Ca 2+ handling proteins ryanodine receptor (RyR) and SERCA2 at day 15 of reprogramming. Densitometric quantification is shown as the average of every replicate, relative to GAPDH. Data are presented as mean +- SD. * p < 0.05, ** p < 0.01, *** p < 0.001.