Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2064 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2064, RRID:AB_1576789
- Product name
- LEFTY1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of LEFTY1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular analysis of LEFTY-expressing cells in early human embryoid bodies.
Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires activation of Smad2/3.
Characterization and mutation analysis of human LEFTY A and LEFTY B, homologues of murine genes implicated in left-right axis development.
Dvash T, Sharon N, Yanuka O, Benvenisty N
Stem cells (Dayton, Ohio) 2007 Feb;25(2):465-72
Stem cells (Dayton, Ohio) 2007 Feb;25(2):465-72
Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires activation of Smad2/3.
Besser D
The Journal of biological chemistry 2004 Oct 22;279(43):45076-84
The Journal of biological chemistry 2004 Oct 22;279(43):45076-84
Characterization and mutation analysis of human LEFTY A and LEFTY B, homologues of murine genes implicated in left-right axis development.
Kosaki K, Bassi MT, Kosaki R, Lewin M, Belmont J, Schauer G, Casey B
American journal of human genetics 1999 Mar;64(3):712-21
American journal of human genetics 1999 Mar;64(3):712-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of LEFTY1 polyclonal antibody (Cat # PAB2064) in HL-60 cell line lysates (35 ug/lane). LEFTY1 (arrow) was detected using the purified Polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinomareacted with LEFTY1 polyclonal antibody (Cat # PAB2064), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)