12411-1-AP

antibody from Invitrogen Antibodies

Targeting: PI4KA PI4K-ALPHA, pi4K230, PIK4CA

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12411-1-AP

Provider

Invitrogen Antibodies

Product name

PI4KA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YLAKFKVKR CGVSELEKEG LRCRSDSEDE CSTQEADGQK ISWQAAIFKV GDDCRQDMLA LQIIDLFKNI FQLVGLDLFV FPYRVVATAP GCGVIECIPD CTSRDQLGRQ TDFGMYDYFT RQYGDESTLA FQQARYNFIR SMAAYSLLLF LLQIKDRHNG NIMLDKKGHI IHIDFGFMFE SSPGGNLGWE PDIKLTDEMV MIMGGKMEAT PFKWFMEMCV RGYLAVRPYM DAVVSLVTLM LDTGLPCFRG QTIKLLKHRF SPNMTEREAA NFIMKVIQSC FLSNRSRTYD MIQYYQNDIP Y (1745-2044 aa encoded by BC018120)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 12411-1-AP (PI4KA antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PI4KA (IP:12411-1-AP, 5ug; Detection:12411-1-AP 1:500) with mouse brain tissue lysate 4000ug.