50-1999-42

antibody from Invitrogen Antibodies

Targeting: CCR9 CDw199, GPR-9-6, GPR28

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

50-1999-42

Provider

Invitrogen Antibodies

Product name

CD199 (CCR9) Monoclonal Antibody (BBC3M4), eFluor™ 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

BBC3M4

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Retinoic acid-induced CCR9 expression requires transient TCR stimulation and cooperativity between NFATc2 and the retinoic acid receptor/retinoid X receptor complex.

Ohoka Y, Yokota A, Takeuchi H, Maeda N, Iwata M
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 15;186(2):733-44

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Efficient induction of CCR9 on T cells requires coactivation of retinoic acid receptors and retinoid X receptors (RXRs): exaggerated T Cell homing to the intestine by RXR activation with organotins.

Takeuchi H, Yokota A, Ohoka Y, Kagechika H, Kato C, Song SY, Iwata M
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5289-99

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Retinoic acid imprints gut-homing specificity on T cells.

Iwata M, Hirakiyama A, Eshima Y, Kagechika H, Kato C, Song SY
Immunity 2004 Oct;21(4):527-38

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TECK, an efficacious chemoattractant for human thymocytes, uses GPR-9-6/CCR9 as a specific receptor.

Youn BS, Kim CH, Smith FO, Broxmeyer HE
Blood 1999 Oct 1;94(7):2533-6

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD4+ T cells isolated from normal human perpheral blood cells were stimulated for 3 days with all-trans retinoic acid, Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). The cells were cultured for 2 additional days with all-trans retinoic acid and Human IL-2 Recombinant Protein (Product # 14-8029-81). These cells were surface stained with Anti-Human/Mouse Integrin beta 7 FITC (Product # 11-5867-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human CD199 (CCR9) eFluor® 660 (right). Total viable cells were used for analysis.