HPA035147

antibody from Atlas Antibodies

Targeting: PHLDB2 FLJ21791, LL5b, LL5beta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA035147

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA035147, RRID:AB_2674492

Product name

Anti-PHLDB2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NPNTLKEGYISVNEINEPCGNSTNLSPSTQFPADA
DAVATEPATAVLASQPQSKEHFRSLEERKKQHKEG
LYLSDTLPRKKTTSSISPHFSSATM


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines U2OS and MCF-7 using Anti-PHLDB2 antibody. Corresponding PHLDB2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-2 OS

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in granular pattern in glandular cells.

Sample type

HUMAN