600-401-B67S

antibody from Rockland Immunochemicals, Inc.

Targeting: RRM2B p53R2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-B67S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-B67S, RRID:AB_10705057

Product name

Anti-Human RRM2B/p53R2 (RABBIT) Antibody - 600-401-B67S

Antibody type

Polyclonal

Vial size

25 µl

References

Submitted references

Ribonucleotide reductase subunit M2B deficiency leads to mitochondrial permeability transition pore opening and is associated with aggressive clinicopathologic manifestations of breast cancer.

Xue L, Liu X, Wang Q, Liu CQ, Chen Y, Jia W, Hsie R, Chen Y, Luh F, Zheng S, Yen Y
American journal of translational research 2018;10(11):3635-3649

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Autophagy induction causes a synthetic lethal sensitization to ribonucleotide reductase inhibition in breast cancer cells.

Chen YR, Tsou B, Hu S, Ma H, Liu X, Yen Y, Ann DK
Oncotarget 2016 Jan 12;7(2):1984-99

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T tranfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1µg/mL and incubated overnight at 4°C. ECL detection was used.

Validation comment

Western Blot