Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [3]
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Validation data
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- Product number
- AF4348 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat TRAM/TICAM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat TRAM/TICAM2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BJQ4
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat TRAM/TICAM2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TRAM/TICAM2 at approximately 31 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRAM/TICAM2 in NRK Rat Cell Line. TRAM/TICAM2 was detected in immersion fixed NRK rat normal kidney cell line using Goat Anti-Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRAM/TICAM2 in Raji Human Cell Line. TRAM/TICAM2 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TRAM/TICAM2 in C2C12 Mouse Cell Line. TRAM/TICAM2 was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TRAM/TICAM2 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TRAM/TICAM2 in NRK Rat Cell Line by Flow Cytometry. NRK rat normal kidney cell line was stained with Goat Anti-Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TRAM/TICAM2 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Goat Anti-Human/Mouse/Rat TRAM/TICAM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4348, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.