PA5-96529

antibody from Invitrogen Antibodies

Targeting: BGLAP OCN

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96529

Provider

Invitrogen Antibodies

Product name

Osteocalcin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: MRALTLLALL ALAALCIAGQ AGAKPSGAES SKGAAFVSKQ EGSEVVKRPR RYLYQWLGAP VPYPDPLEPR REVCELNPDC DELADHIGFQ EAYRRFYGPV

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Mechanics-driven nuclear localization of YAP can be reversed by N-cadherin ligation in mesenchymal stem cells.

Zhang C, Zhu H, Ren X, Gao B, Cheng B, Liu S, Sha B, Li Z, Zhang Z, Lv Y, Wang H, Guo H, Lu TJ, Xu F, Genin GM, Lin M
Nature communications 2021 Oct 28;12(1):6229

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Osteocalcin was performed in C6 cells using Osteocalcin Polyclonal Antibody (Product # PA5-96529) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Osteocalcin was performed in U-2 OS cells using Osteocalcin Polyclonal Antibody (Product # PA5-96529) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Osteocalcin in paraffin-embedded mouse stomach using Osteocalcin Polyclonal Antibody (Product # PA5-96529) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Osteocalcin in paraffin-embedded human stomach using Osteocalcin Polyclonal Antibody (Product # PA5-96529) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Osteocalcin in paraffin-embedded mouse lung using Osteocalcin Polyclonal Antibody (Product # PA5-96529) at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 The HAVDI/RGD hydrogel can be used to reduce long-term retention of the effects of mechanotransduction and inhibit osteogenic differentiation of hMSCs. a Schematic of the experimental protocol for assessing osteogenic differentiation of hMSCs cultured on TCP for 1-7 d, followed by 3 d (for RUNX2) or 7 d (for OCN and ALP) culture on 20 kPa Scram/RGD or HAVDI/RGD hydrogels. hMSCs were cultured on TCP (dark purple) in growth medium (pink) for 1, 3, or 7 d and then transferred (light yellow, day 0) to soft Scram/RGD (orange) or HAVDI/RGD (blue) hydrogels for additional 3 d or 7 d of culture in osteogenic medium (light blue) before collection and analysis (gray, day 3 or day 7). RUNX2 expression was assessed in the conditions denoted So3 (soft control, without culture on TCP), DT1 + So3, DT3 + So3, or DT7 + So3. OCN and ALP expression were assessed in the conditions denoted So7 (soft control, without culture on TCP), DT1 + So7, DT3 + So7, or DT7 + So7. b Representative confocal images of cells stained for RUNX2, a marker of early osteogenic differentiation, in hMSCs following treatment as shown in a . Scale bars, 20 um. c Analysis of confocal images showed a significant increase in the RUNX2 n/c ratio with increasing exposure to TCP in hMSCs transferred to Scram/RGD hydrogels (from left to right n = 77, 49, 59, 73, 72, 44, 84, 92 cells examined over 7, 15, 19, 20, 12, 24, 23, 26 images respectively, p -values were obtained using one-way ANOVA followed by Tukey's post hoc te