sc-20121

antibody from Santa Cruz Biotechnology

Targeting: MIF GIF, GLIF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-20121

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-20121, RRID:AB_648587

Product name

Anti-MIF

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

A role for NRAGE in NF-kappaB activation through the non-canonical BMP pathway.

Matluk N, Rochira JA, Karaczyn A, Adams T, Verdi JM
BMC biology 2010 Jan 25;8:7

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Macrophage migration inhibitory factor is a direct target of HBP1-mediated transcriptional repression that is overexpressed in prostate cancer

Y C Chen, X W Zhang, X H Niu, D Q Xin, W P Zhao, Y Q Na, Z B Mao
Oncogene 2010 Apr;29(21):3067-3078

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 12

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in Leydig cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.