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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- PB9274 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MIF Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for MIF detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. MIF information: Molecular Weight: 12476 MW; Subcellular Localization: Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- MIF Picoband antibody, PB9274, Western blottingAll lanes: Anti MIF (PB9274) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: HUT Whole Cell Lysate at 40ugLane 4: A549 Whole Cell Lysate at 40ugLane 5: HEPG2 Whole Cell Lysate at 40ugPredicted bind size: 12KDObserved bind size: 12KD
- Additional image
